| 海南不同地理群体羊鲍18SrDNA的克隆与序列分析 |
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| 引用本文： | 杨文杰,黄勃,王仁恩,张钰.海南不同地理群体羊鲍18SrDNA的克隆与序列分析[J].安徽农业科学,2012,40(20):10370-10373. |
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| 作者姓名： | 杨文杰 黄勃 王仁恩 张钰 |
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| 作者单位： | 海南大学海洋学院,海南海口,570228;海南大学海洋学院,海南海口,570228;海南大学海洋学院,海南海口,570228;海南大学海洋学院,海南海口,570228 |
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| 基金项目： | 海南大学植物学国家重点学科，国家“863”资源环境技术领域项目，国家自然科学基金，教育部科学技术研究重点项目，海南省重大科研项目，海南大学重大科研项目 |
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| 摘 要： | 目的]对海南不同地理群体羊鲍18S rDNA进行克隆,并对其进行序列分析。方法]采用分子生物学的方法,对海南不同海区的2个羊鲍地理群体18S rRNA基因全长进行克隆和序列分析,并将得到的羊鲍18S rRNA基因序列与同海域耳鲍的进行比较。结果]同一地理群体内羊鲍核糖体18S rRNA基因序列完全一致;不同地理群体间羊鲍核糖体18S rRNA基因在碱基组成上的相似率为99.5%,仅在某些位点处发生了碱基替换,即腺嘌呤(T)被鸟嘌呤(G)替换;同时,将这两个不同群体中羊鲍的18S rRNA基因与同一海域耳鲍18S rRNA基因序列进行比较分析发现,它们之间也只是发生了碱基替换。结论]为海南鲍鱼特别是羊鲍的遗传多样性、遗传结构、种质鉴定及其种质资源的保护和利用等方面的研究提供可靠依据。
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| 关 键 词： | 地理群体 羊鲍 18S rRNA基因 |
Cloning and Sequence Analysis of Haliotis ovina 18S rDNA in the Different Geographical Populations of Hainan |
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| Institution: | YANG Wen-jie et al(College of Ocean,Hainan University,Haikou,Hainan 570228) |
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| Abstract: | Objective] The aim was to clone and analyze the sequence of 18S rDNA from Haliotis ovina.Method] The 18S rRNA genes of two different H.ovina geographical populations,which from different area of Hainan,were cloned by molecular biology method and sequenced,and then aligned with H.asinina 18S rRNA genes of same sea area.Result] 18S rRNA genes of individuals from the same group had no differentiation,the similarity in the 18S rRNA genes neared 100%,whereas partial differentiation between the 2 groups was observed with the similarity up to 99.5%,and basal substitution took place at some sites.A neighbor-joining tree was constructed from sequence divergence of 18S rRNA genes,which all was built up by ocated accurately those sequences according to species of abalone.An unweighted pair-group dendrogram method with arithmetic mean was constructed from divergence among the individuals from the 2 groups.Conclusion] It prepared reliable basis for the genetic diversity,hereditary constitution,germplasm identification,the conservation and utilization of germplasm resource and other aspects of the study of Hainan abalone,H.ovina specially. |
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| Keywords: | Geographic group Haliotis ovina 18S rRNA gene |
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