| 黄姜皂素生产废渣固态发酵纤维素酶产酶条件优化及酶学特性初探 |
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| 引用本文： | 李发永,呼世斌,郑志伟,韩丰波,贺小荣.黄姜皂素生产废渣固态发酵纤维素酶产酶条件优化及酶学特性初探[J].西北农业学报,2007,16(6):277-281. |
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| 作者姓名： | 李发永 呼世斌 郑志伟 韩丰波 贺小荣 |
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| 作者单位： | 西北农林科技大学资源与环境工程学院,陕西杨凌,712100 |
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| 摘 要： | 以黄姜皂素生产废渣为原料,考察了不同培养条件对木霉产纤维素酶的影响,并进行了酶学性质的初步研究。结果表明,表面活性剂吐温-80添加量为0.08%时能显著提高酶活力;一定量的碳酸钙能显著提高酶活力,其最适添加量为0.5%;通过正交试验找出了最佳的培养条件,CMC酶活最佳培养条件为：培养温度28℃,培养4 d,起始pH5.0。滤纸（FPA）酶活为：培养温度28℃,培养5 d,pH5.0。该酶的最适反应温度为60℃,最适反应pH为4.5,其热稳定性不好,金属离子对其酶活具有抑制作用。
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| 关 键 词： | 皂素生产废渣 固态发酵 产酶条件 酶学特性 |
| 文章编号： | 1004-1389（2007）06-0277-05 |
| 收稿时间： | 2007-06-13 |
| 修稿时间： | 2007-07-09 |
Preliminary Study on Optimization of Enzyme Solid-fermentation Conditions and Properties of Enzymology by Utilization of Diosgenin Solid Waste |
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| LI Fa-yong,HU Shi-bin,ZHENG Zhi-wei,HAN Feng-bo and HE Xiao-rong.Preliminary Study on Optimization of Enzyme Solid-fermentation Conditions and Properties of Enzymology by Utilization of Diosgenin Solid Waste[J].Acta Agriculturae Boreali-occidentalis Sinica,2007,16(6):277-281. |
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| Authors: | LI Fa-yong HU Shi-bin ZHENG Zhi-wei HAN Feng-bo and HE Xiao-rong |
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| Institution: | College of Resources and Environment, Northwest A & F University, Yangling Shaanxi 712100, China;College of Resources and Environment, Northwest A & F University, Yangling Shaanxi 712100, China;College of Resources and Environment, Northwest A & F University, Yangling Shaanxi 712100, China;College of Resources and Environment, Northwest A & F University, Yangling Shaanxi 712100, China;College of Resources and Environment, Northwest A & F University, Yangling Shaanxi 712100, China |
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| Abstract: | |
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| Keywords: | Diosgenin solid waste Solid- fermentation Enzyme production conditions Characteristic of enzymology |
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