An assessment of cultivated emmer germplasm for gluten proteins |
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| Authors: | A M Degaonkar S A Tamhankar V S Rao |
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| Institution: | (1) Agharkar Research Institute, Agarkar Road, Pune, 411004, India |
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| Abstract: | The storage protein composition of 61 accessions of Triticum dicoccum was analyzed by SDS-PAGE (HMW- and LMW-glutenin subunits) and Acid-PAGE (gliadins). In the HMW-glutenin subunits, four allelic
variants at the Glu-A1 and eight at the Glu-B1 locus were detected resulting in a total of 17 patterns. The Glu-B1 locus was found to be more polymorphic than the Glu-A1 locus. Interestingly, the presence of HMW subunits like 13+16, 2∗ and 1 associated with good quality was observed. Three accessions were null for both the Glu-A1 and Glu-B1 loci. There was less variation for gliadins. Three different gamma gliadin fractions coded by Gli-B1 locus were detected and there were eight different LMW-B glutenin patterns at the Glu-3 loci. The variability can be used to improve the utility of this crop. |
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| Keywords: | Acid-PAGE cultivated emmer gliadins glutenins SDS-PAGE Triticum dicoccum |
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