| 一株放线菌发酵产物抗肿瘤活性的研究 |
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| 引用本文： | 郑丰平,何开泽,蒲蔷.一株放线菌发酵产物抗肿瘤活性的研究[J].安徽农业科学,2009,37(27):12896-12897. |
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| 作者姓名： | 郑丰平 何开泽 蒲蔷 |
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| 作者单位： | 中国科学院成都生物研究所,四川成都,610041;中国科学院成都生物研究所,四川成都,610041;中国科学院成都生物研究所,四川成都,610041 |
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| 摘 要： | 目的]研究一株放线茵发酵产物的抗肿瘤活性。方法]采用HepG2作为肿瘤细胞模型进行抗肿瘤活性检测。结果]用石油醚、乙酸乙酯、正丁醇依次萃取发酵液离心后的沉淀和上清液，分别得到6个浸膏样品。发现用乙酸乙酯萃取上清液得到的5号样品抗肿瘤活性最高，在没有分离纯化的情况下G1 50达到0．76μg,／ml。对5号样品进行分离后得到物质A，在其浓度为10．00μg／ml时，对肿瘤细胞抑制率为26．50％。结论]此株放线菌发酵产物具有高效抗肿瘤活性，可以进行深入研究。
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| 关 键 词： | 放线菌 发酵 抗肿瘤 活性检测 细胞毒性 |
Research on the Activity of the Products Fermented with Actinomycete in Anti-tumor |
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| ZHENG Feng-ping et al.Research on the Activity of the Products Fermented with Actinomycete in Anti-tumor[J].Journal of Anhui Agricultural Sciences,2009,37(27):12896-12897. |
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| Authors: | ZHENG Feng-ping |
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| Institution: | ZHENG Feng-ping et al(Institute of Biology,Chinese Academy of Sciences,Chengdu,Sichuan 610041) |
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| Abstract: | Objective] The anti-tumor activity of a fermentation product of actinomycosis was studied.Method] HepG2 tumor cells ere used as a model for the detection of anti-tumor activity.Results] The precipitate and supernatant of the fermentation solution after the centrifugation were extracted with the petroleum ether,ethyl acetate and n-butanol in order,respectively,and 6 samples were obtained.The result indicated that the supernatant from the Sample No.5 extracted with ethyl acetate had the highest anti-tumor ... |
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| Keywords: | Actinomycosis Fermentation Anti-tumor Activity detection Cytotoxicity |
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