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Mapping of Fertility Restoring Gene for Aegilops kotschyi Cytoplasmic Male Sterility in Wheat Using SSR Markers |
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| Authors: | LIU Bao-shen SUN Qi-xin GAO Qing-rong SUN Lan-zhen XIE Chao-jie LI Chuan-you NI Zhong-fu DOU Bing-de |
| Institution: | 1. Agronomy Department, Shandong Agricultural University, Taian 271018, P. R. China;Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100094, P.R.China 2. Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100094, P.R.China 3. Agronomy Department, Shandong Agricultural University, Taian 271018, P. R. China |
| Abstract: | LK783 was found to be a good fertility restorer for K-type male sterility of wheat. Microsatellite markers were employed to map the major restoring gene in LK783. Maintainer and restorer DNA pools were established using the extreme sterile and fertile plants among (KJ5418A//911289/LK783)F1 population,respectively. Seventy-nine sets of SSR primers were screened for polymorphism between the two pools, 6 of which were found polymorphic. Linkage analysis showed that Xgwm11, Xgwm18 , Xgwm264a and Xgwm273were linked to the restoring gene in LK783, while Xgwm11, Xgwm18 and Xgwm273 were co-segregated. The distance between the Rf gene in LK783 and the three co-segregated markers was 6.54±4.37 cM, the distance between Rf gene and Xgwm264a was 5.71±4.10 cM. The four SSR markers were located on chromosome 1BS by amplifying the DNA of nulli-tetrasomics and ditelosomics of CS with the 4 sets of primers, indicating that the major restoring gene in LK783 was located on 1BS, but the relative location of the gene was different from Rfv1, allelism of the two genes should be further investigated. The breeding for new fertility restorer lines of K-type cytoplasmic male sterility in wheat would be facilitated by using the four polymorphic markers. |
| Keywords: | Wheat ( Triticum aestivum ) Cytoplasmic male sterility Restoring gene Molecular marker |
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