| 兔胚胎发育的渗透体积研究 |
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| 引用本文： | 姜国诚,孙艳香,秦鹏春.兔胚胎发育的渗透体积研究[J].浙江大学学报(农业与生命科学版),1996(1). |
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| 作者姓名： | 姜国诚 孙艳香 秦鹏春 |
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| 作者单位： | 浙江农业大学动物科学学院，东北农业大学 |
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| 摘 要： | 利用显微镜扩散室研究兔胚胎１～１６细胞的渗透静止体积和渗透平衡体积，以及他们与胚胎发育的关系。兔的１、２、４、８和１６细胞的渗透静止体积分别是０．２３５、０．２１１、０．１９３、０．１６９和０．１４８．结果表明，在１６细胞以前，随着胚胎的发育，渗透静止体积变小，干物质和结合水的含量不断下降。同时，低温保护剂的浓度增大，渗透平衡体积则变小，更接近于渗透静止体积。乙二醇的渗透能力强于ＤＭＳＯ（二甲基亚砜），在乙二醇溶液中，渗透平衡体积要大于在ＤＭＳＯ中。
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| 关 键 词： | 渗透静止体积 渗透平衡体积 胚胳发育 兔 |
Studies on the osmotic volumes of rabbit emhryo development |
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| Jiang Guocheng,Sun
Yanxiang,Qin Pengchun.Studies on the osmotic volumes of rabbit emhryo development[J].Journal of Zhejiang University(Agriculture & Life Sciences),1996(1). |
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| Authors: | Jiang Guocheng Sun
Yanxiang Qin Pengchun |
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| Abstract: | cell.2-cell.4-cell,8-cell and 16.cell rabbit embryos were collected by superovula-tion. The
microscopic diffusion chamber was made to observe and study the osmotic inactive vol-umes
and osmotic equilibrium volumes of embryos and their osmotic behaviors with their
develop-ment.The osmotic inactive volumes of 1-cell,2-cell,4-cell, 8-cell and 16-cell embryos
are 0. 235,0.211,0.193,0.169 and 0.148,respectively.The results show the osmotic inactive
volumes become smaller,which means dry substances and bound water in embryos at early
stage decrease with their development. When concentrations of protectants increase,the
osmotic equilibrium volulnes of em-bryos decrease, more close to osmotic inactive
volumes;Glycol has higher permeability than DM-SO. |
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| Keywords: | osmotic inactive volume osmotic equilibrium volume embryo development rabbit |
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