| 新型化合物唑嘧氯草胺的作用机制 |
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| 引用本文： | 杨娜,王金信,刘伟堂,金涛,沈德隆.新型化合物唑嘧氯草胺的作用机制[J].植物保护学报,2010,37(6):562-566. |
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| 作者姓名： | 杨娜 王金信 刘伟堂 金涛 沈德隆 |
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| 作者单位： | 1. 山东农业大学植物保护学院,泰安,271018
2. 浙江工业大学,杭州,310014 |
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| 摘 要： | 采用培养皿法和常规生化方法,研究了新型除草剂唑嘧氯草胺(暂定名,代号:ZJ-2725)的作用机制。结果显示,同时添加20mg/L浓度的缬氨酸、亮氨酸和异亮氨酸能完全恢复唑嘧氯草胺对苘麻芽的生长抑制作用,而添加相同浓度的单一支链氨基酸只能部分消除其抑制作用。离体条件下,随着唑嘧氯草胺浓度的增大,对乙酰乳酸合成酶(ALS)活性的抑制率增加,ALS活性随反应时间的延长而增加,且两者之间符合Michaelis-Meten方程;活体条件下,唑嘧氯草胺对苘麻的ALS也表现出明显的抑制作用,ALS活性明显下降。表明唑嘧氯草胺在植物体内抑制了3种支链氨基酸的生物合成,进而导致植物蛋白质合成受阻而使植物生长受到抑制,ALS即为唑嘧氯草胺的作用靶标。
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| 关 键 词： | 作用机制 唑嘧氯草胺 支链氨基酸 乙酰乳酸合成酶 |
| 收稿时间： | 3/9/2010 12:00:00 AM |
Effect mechanism of a novel compound ZJ-2725 |
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| Yang N,Wang Jinxin,Liu Weitang,Jin Tao and Shen Delong.Effect mechanism of a novel compound ZJ-2725[J].Acta Phytophylacica Sinica,2010,37(6):562-566. |
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| Authors: | Yang N Wang Jinxin Liu Weitang Jin Tao and Shen Delong |
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| Institution: | College of Plant Protection, Shandong Agricultural University, Tai'an 271018, Shandong Province, China;College of Plant Protection, Shandong Agricultural University, Tai'an 271018, Shandong Province, China;College of Plant Protection, Shandong Agricultural University, Tai'an 271018, Shandong Province, China;College of Plant Protection, Shandong Agricultural University, Tai'an 271018, Shandong Province, China;Zhejiang University of Technology, Hangzhou 310014, Zhenjiang Province, China |
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| Abstract: | Using Petri dish method and normal biochemical methods, the mechanism of a novel compound ZJ-2725 was studied under the laboratory condition. The results showed the inhibition of ZJ-2725 on the stem of Abutilon theophrasti could recover when adding three branched chain amino acids valine, leucine, and isoleucine together at 20 mg/L concentration. However, it could not eliminate the inhibition effect absolutely when only adding one of the three branched chain amino acids at the same concentration. In vitro, the inhibition rate on the specific activity of acetolaetate synthase (ALS) increased with the concentration increase of ZJ-2725. The specific activity of ALS increased as the reaction time was prolonged, and the relationship between the specific activity of ALS and the reaction time was coincident with Michaelis-Meten equation. In vivo, ZJ-2725 also inhibited the specific activity of ALS. The results revealed that ZJ-2725 could prevent biosynthesis of the three branched chain amino acids, then restrained the pathway of protein biosynthesis, and hence inhibited the plant growth, indicating that ZJ-2725 was the inhibitor of ALS. |
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| Keywords: | effect mechanism ZJ-2725 branched chain amino acid acetolactate synthase |
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