| 炎毒热清及其微乳制剂小鼠急性毒性试验和体外抑菌作用 |
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| 引用本文： | 王棋文,李剑勇,牛建荣,周旭正,张继瑜,魏小娟,杨亚军,赵兴绪.炎毒热清及其微乳制剂小鼠急性毒性试验和体外抑菌作用[J].安徽农业科学,2008,36(36). |
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| 作者姓名： | 王棋文 李剑勇 牛建荣 周旭正 张继瑜 魏小娟 杨亚军 赵兴绪 |
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| 作者单位： | 1. 甘肃农业大学动物医学院,甘肃兰州,730070
2. 中国农业科学院兰州畜牧与兽药研究所新兽药工程重点实验室,甘肃兰州,730050 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金资助项目，甘肃省支撑计划项目 |
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| 摘 要： | 目的]为评价炎毒热清(AEE)及其微乳制剂(AEE-ME)的安全性和抑菌活性。方法]用灌胃法对小鼠进行急性毒性试验,并采用改良寇氏法计算半数致死量及其95%可信区间;采用杯碟法研究抑菌作用。结果]AEE经小鼠口服LD50为10.937 g/kg,95%可信区间为9.309~12.850 g/kg;AEE-ME经小鼠口服LD50为5.357 g/kg,95%可信区间为4.388~6.566 g/kg。AEE对猪大肠杆菌O149、金黄色葡萄球菌、鸡白痢沙门氏菌、无乳链球菌的MIC分别为10.00、20.00、20.00、10.00 mg/ml;AEE-ME对上述4种菌的MIC依次为5.00、10.00、5.00、5.00 mg/ml,对绿脓杆菌的MIC为20.00 mg/ml。结论]AEE属实际无毒性物质,AEE-ME为低毒性物质,二者均有抑菌活性且AEE-ME抑菌作用强于AEE。
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| 关 键 词： | 炎毒热清 微乳 半数致死量(LD50) 抑菌活性 |
The Acute Toxicity Test in Mice and Bacteriostasis in vitro of Yandureqing and Its Microemulsion |
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| Abstract: | Objective] The aim of this study is to evaluate the safety and bacteriostasis of Yandureqing(AEE) and its microemulsion(AEE-ME).Method] The acute toxicity was tested in mice by intragastric administration,and median lethal dose(LD50) as well as its 95% confidence interval was calculated by modified Karber method;the bacteriostasis was investigated by cylinder plate method.Result] LD50 of AEE in mice was 10.937 g/kg with the 95% confidence interval of 9.309-12.850 g/kg;and LD50 of AEE-ME in mice was 5.357 g/kg with the 95% confidence interval of 4.388-6.566 g/kg.The MICs of AEE to Escherichia coli O149 from swine,Staphylococcus aureus,Salmonella pullorum and Streptococcus agalactiae were 10.00,20.00,20.00 and 10.00 mg/ml,respectively;while the MICs of AEE-ME to the 4 kinds of bacteria mentioned above were 5.00,10.00,5.00 and 5.00 mg/ml in turn,and that to Pseudomonas aeruginosa was 20.00 mg/ml.Conclusion] AEE is an actually nontoxic drug and AEE-ME belongs to the low toxic preparation.AEE and AEE-ME have obvious bacteriostasis,in which AEE-ME is superior to AEE. |
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| Keywords: | Yandureqing Microemulsion Median lethal dose(LD50) Bacteriostasis |
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