| 卷丹百合脱毒快繁技术研究 |
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| 引用本文： | 周晓波,吴艺飞,丁茁荑.卷丹百合脱毒快繁技术研究[J].中国农学通报,2012,28(31):201-205. |
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| 作者姓名： | 周晓波 吴艺飞 丁茁荑 |
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| 作者单位： | 1. 湖南省蔬菜研究所,长沙410125;湖南省蔬菜工程技术研究中心,长沙410125
2. 湖南省蔬菜研究所,长沙410125;湖南省蔬菜工程技术研究中心,长沙410125;湖南农业大学园艺园林学院,长沙410128 |
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| 基金项目： | 湖南省蔬菜研究所青年创新基金“卷丹百合脱毒快繁技术研究”(hnsscyjs2011qncx06) |
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| 摘 要： | 对卷丹百合脱毒苗组培快繁技术体系进行研究。以带LSV病毒的卷丹百合珠芽为材料,通过36℃高温预处理10天,剥取0.2mm大小的茎尖在MS＋2.5mg/L6-BA＋1.5mg/LGA＋0.5mg/LNAA＋0.1g/L活性炭＋0.6%琼脂＋3%白糖的培养基中进行芽诱导培养,经过一次继代培养后,用RT-PCR检测LSV病毒,脱毒率达100%。将脱毒百合苗在MS＋2.0mg/L6-BA＋2.0mg/L2,4-D＋0.1mg/LNAA培养基中诱导愈伤组织及丛生芽,诱导率达100%;在1/2MS＋2.5mg/L6-BA＋0.2mg/LNAA培养基中进行分化、增殖培养,增殖倍数达到4.31;采用MS＋1.2mg/LNAA＋0.5g/L活性炭培养基进行生根培养,产生的根系多而粗壮,移栽时最易成活。
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| 关 键 词： | 烟台市 烟台市 |
| 收稿时间： | 3/8/2012 12:00:00 AM |
| 修稿时间： | 2012/5/10 0:00:00 |
Study on the Detoxification and Rapid Propagation of Lilium lancifolium Thunb. |
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| Zhou Xiaobo
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Wu Yifei
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Ding Zhuoyi.Study on the Detoxification and Rapid Propagation of Lilium lancifolium Thunb.[J].Chinese Agricultural Science Bulletin,2012,28(31):201-205. |
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| Authors: | Zhou Xiaobo Wu Yifei Ding Zhuoyi |
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| Institution: | 1,2(1 Hunan Vegetable Research Institute,Changsha 410125;2 Hunan Vegetable Research and Development Center,Changsha 410125;3 Horticulture and Landscape College,Hunan Agricultural University,Changsha 410128) |
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| Abstract: | In this work,Lilium lancifolium bulbils with LSV viruses were used as experiment material.Stripping 0.2 mm size of the shoot apex after 36℃ pretreatment 10 d,then culturing them in MS ＋ 2.5 mg/L 6-BA＋1.5 mg/L GA ＋0.5 mg/L NAA ＋0.1 g/L activated carbon ＋0.6% agar ＋3% sugar for bud induction,and detecting LSV virus using RT-PCR after a subculture,we found the virus-free rate to be 100%.The virus-free lily seedlings were put in MS ＋2.0 mg/L 6-BA ＋2.0 mg/L 2,4-D ＋0.1 mg/L NAA culture medium to induce callus and adventitious bud,induction rate 100%;in 1/2MS ＋2.5 mg/L 6-BA ＋0.2 mg/L NAA culture medium for differentiation and proliferation culture,proliferation multiples of Lilium lancifolium bulbils up to 4.31;in MS＋1.2 mg/L NAA＋0.5 g/L activated carbon culture medium for rooting,roots stout and branching vigorously,easy to survive after transplanting. |
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| Keywords: | Lilium lancifolium Thunb shoot tip detoxification rapid propagation |
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