Effect of VP28 DNA vaccine on white spot syndrome virus in <Emphasis Type="Italic">Litopenaeus vannamei</Emphasis> |
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| Authors: | Xiang Li Qing-hui Liu Lin Hou Jie Huang |
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| Institution: | (1) Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 266071 Qingdao, People’s Republic of China;(2) Department of Biology, LiaoNing Normal University, 116029 DaLian, LiaoNing, China; |
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| Abstract: | White spot syndrome virus (WSSV) occurs worldwide and causes high mortality and considerable economic damage to the shrimp
farming industry. Considering the global environmental, the economic and sociological importance of shrimp farming, and the
constraints of high intensity cultivation, development of novel control measures against the outbreak of WSSV become inevitable.
In this study, we have explored the protective efficacy of DNA vaccination and tissue distribution of the recombinant plasmid
in immunized Litopenaeus vannamei. The VP28 gene was cloned in the eukaryotic expression vector pVAX1, and the construct vector was named as lpv28. The protective
effect of lpv28 against WSSV was evaluated in L. vannamei by injecting lpv28 construct and later challenging with WSSV. Expression of these proteins from the recombinant plasmids
was confirmed in vitro by RT-PCR and Western blot analysis. The result of vaccination trials showed that a survival rate in
shrimp vaccinated with lpv28 was 52.5% at most compared to control groups (100% mortality). The immunological parameters analyzed
in the vaccinated and control groups showed that the vaccinated groups owned a high level of lysozyme, alkaline phosphatase,
and total superoxide dismutase when compared to the control group. Furthermore, protein expression analysis indicated that
VP28 can be detected in gill, muscle and head soft tissue of the shrimps in the immunized group after 14th day injection.
Thus, the result indicated that DNA vaccination strategy has a potential utility against WSSV. |
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