Isolation and characterization of a potyvirus associated with bushy dwarf symptom in chickpea, Cicer arietinum, in India |
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| Authors: | V ANJAIAH D V R REDDY S K MANOHAR R A NAIDU Y L NENE A S RATNA |
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| Institution: | Legumes Program, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, A.P. 502 -324. India |
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| Abstract: | A potyvirus that induced stunting and a characteristic bushy appearance at the apical region, due to proliferation of terminal branches with narrowed, reduced and deformed leaflets, was isolated from chickpea in India. The virus was sap-transmissible to 14 species of Chenopodiaceae, Leguminosae, Solanaceae and Malvaceae; Chenopodium amaranticolor was a good local lesion host. Virus particles, trapped by immunosorbent electron microscopy and stained with uranyl acetate, were 710 ×10 nm long. Purified virus preparations contained a single polypeptide species of 32,500 Da and one nucleic acid species of 3.1 · 106 Da. The virus was serologically related to soybean mosaic, azuki bean mosaic and peanut mottle viruses but not to clover yellow vein, pea seed-borne mosaic and bean yellow mosaic viruses.
On the basis of these properties, the virus was identified as a previously undescribed potyvirus in chickpea, for which the name chickpea bushy dwarf virus is proposed. |
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