| 一个花生早期胚特异性表达基因AhDGAT3启动子的克隆及功能分析 |
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| 引用本文： | 石磊,齐飞艳,苗利娟,黄冰艳,刘华,张忠信,高伟,董文召,汤丰收,张新友.一个花生早期胚特异性表达基因AhDGAT3启动子的克隆及功能分析[J].中国油料作物学报,2018,40(1):25. |
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| 作者姓名： | 石磊 齐飞艳 苗利娟 黄冰艳 刘华 张忠信 高伟 董文召 汤丰收 张新友 |
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| 作者单位： | 河南省农业科学院经济作物研究所／农业部黄淮海油料作物重点实验室／河南省油料作物遗传改良重点实验室/花生遗传改良国家地方联合工程实验室，河南 郑州 450002 |
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| 基金项目： | 国家高技术研究与发展计划（863计划）（2013AA102602-6）；河南省重大科技专项（161100111000）；国家现代农业产业技术体系项目（CARS-13）；河南省现代农业产业技术体系项目(S2012-5) |
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| 摘 要： | 为获得在早期胚中特异性表达启动子，本研究通过基因组步移技术对AhDGAT3（GenBank: AY875644.1）的启动子进行克隆。研究结果表明，获得了AhDGAT3 起始密码子ATG上游 5´侧翼序列2181 bp，应用PLACE和plantCARE在线分析显示，AhDGAT3启动子除了含有核心调控元件TATA-box和CAAT-box之外，还有多个非生物胁迫作用元件，如茉莉酸响应元件、防御和干旱胁迫响应元件TC-rich repeats、光响应元件、干旱诱导的MYB结合位点、光响应MYB结合位点等之外，还包含种子表达相关顺式作用元件及分裂组织表达相关元件。构建重组载体pBI121-PAhDGAT3，转化拟南芥，GUS染色结果显示，该启动子能驱动下游GUS基因，仅在发育早期的心型胚期至鱼雷型胚期较短时间内的胚中特异性表达。
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| 关 键 词： | 花生 DGAT3 早期胚 种子特异性启动子 GUS报告基因 |
Cloning and functional analysis of a novel early embryo-specific expressed AhDGAT3 promoter from Arachis hypogaea L. |
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| SHI Lei,QI Fei-yan,MIAO Li-juan,HUANG Bing-yan,LIU Hua,ZHANG Zong-xin,GAO Wei,DONG Wen-zhao,TANG Feng-shou,ZHANG Xin-you.Cloning and functional analysis of a novel early embryo-specific expressed AhDGAT3 promoter from Arachis hypogaea L.[J].Chinese Journal of Oil Crop Sciences,2018,40(1):25. |
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| Authors: | SHI Lei QI Fei-yan MIAO Li-juan HUANG Bing-yan LIU Hua ZHANG Zong-xin GAO Wei DONG Wen-zhao TANG Feng-shou ZHANG Xin-you |
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| Institution: | Industrial Crops Research Institute, Henan Academy of Agricultural Sciences / Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry of Agriculture, P R China / Henan Provincial Key Laboratory for Oil Crops Improvement / National and Provincial Joint Engineering Laboratory for Peanut Genetic Improvement, Henan Province, Zhengzhou 450002, China |
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| Abstract: | To characterize an early embryo-specific promoter, the putative promoter region of AhDGAT3 (GenBank: AY875644.1) was isolated by nested PCR-based genomic walking method. Results showed that the 2181 bp 5´ flanking sequence of AhDGAT3 was isolated. Sequence analysis in PLACE and plantCARE database identified the core regulatory elements such as TATA-box and CAAT-box， the putative cis-acting regulatory elements involved in MeJA-responsiveness, defense stress responsiveness, light-responsiveness, heat stress responsiveness and further the seed or embryo-specific elements. For the functional and characteristic analysis of AhDGAT3 promoter, the recombinant vector pBI121-PAhDGAT3 was designated and transformed into Arabidopsis. Histochemical staining revealed that the activity of pAhDGAT3 was restricted to a short temporal and spatial window from the heart stage to torpedo stage of the embryo. |
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| Keywords: | Peanut (Arachis hypogaea L ) DGAT3 Early embryo Seed-speci?c promoter GUS reporter gene |
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