| 不同大豆种质种子耐浸水能力鉴定 |
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| 引用本文： | 陈文杰,梁江,汤复跃,韦清源,郭小红,陈渊.不同大豆种质种子耐浸水能力鉴定[J].中国油料作物学报,2018,40(3):381. |
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| 作者姓名： | 陈文杰 梁江 汤复跃 韦清源 郭小红 陈渊 |
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| 作者单位： | 广西农业科学院经济作物研究所，广西农业科学院玉米研究所，广西 南宁，530007 |
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| 基金项目： | 国家大豆产业技术体系资助项目（CARS-04-CES30）；广西农科院团队项目（2015YT59）；广西重点研发计划（桂科AB16380097）；广西农科院基本科研项目专项（2015YM21）；广西农科院科技发展基金项目（2015JZ111） |
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| 摘 要： | 播种后遭遇渍害是南方夏大豆生产面临的主要逆境危害之一。为研究不同大豆种质播后对浸水耐性差异表现，以种子浸种120h模拟渍害条件，测定137份大豆材料浸泡液电导率，并进行标准发芽试验，统计种子发芽势（GPP）、发芽率（GP）、正常苗率（NSP）等指标，并利用主成分分析、聚类分析等分析方法对多个指标进行多元统计分析，利用综合耐浸水指标及简易指标对大豆种子耐浸水能力进行等级划分，筛选耐浸水能力强的大豆材料。结果表明，浸水处理120h后，GPP、GP、NSP、轻微损伤粒率（SISP）、严重损伤粒率（IBSP）、烂粒率（DSP）及电导率等6个指标的变异系数均大于50%，GPP、GP和NSP同大豆百粒重呈显著负相关，电导率同百粒重呈显著正相关。利用主成分分析提取两个主成分：“健康因子”和“过渡因子”，贡献率分别为64.45%和15.83%，根据两因子得分矩阵进行聚类分析，可将供试材料划分为3类。利用综合指标法将137份大豆材料划分为5种耐浸水级别，表现耐浸水的大豆材料9份，并在GP、GPP、NSP三个简易耐浸水指标筛选中同时表现耐浸水的大豆材料5份，可作为进一步筛选鉴定的对照种质资源。大豆浸泡液电导率与种子正常苗率相结合可作为大批量筛选耐浸水种质的指标。
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| 关 键 词： | 大豆 耐浸水 渍害 种质资源 |
Analysis of seed-flooding tolerance of soybean germplasms |
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| CHEN Wen-jie,LIANG Jiang,TANG Fu-yue,WEI Qing-yuan,GUO Xiao-hong,CHEN Yuan.Analysis of seed-flooding tolerance of soybean germplasms[J].Chinese Journal of Oil Crop Sciences,2018,40(3):381. |
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| Authors: | CHEN Wen-jie LIANG Jiang TANG Fu-yue WEI Qing-yuan GUO Xiao-hong CHEN Yuan |
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| Institution: | Cash Crops Research Institute, Maize Research Institute, Guangxi Academy Of Agricultural Sciences, Nanning 530007, China |
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| Abstract: | Seed-flooding is one of the most important adversity stress in south summer soybean production in China. For researching the seed-flooding tolerance difference of soybean germplasms, 137 materials were treated for 120 hours simulating the field seed-flooding condition. The electrical conductivity of soybean soaking liquid was determined. Germination potential percentage (GPP), germination percentage (GP), normal seeding percentage (NSP), slightly injured seedling percentage (SISP), injured badly seedling percentage (IDSP) was counted through standard germination test. Multivariate statistical analysis, such as principal component analysis and cluster analysis, were used. Comprehensive and sample seed-flooding tolerance index were used to classify the soybean germplasms. Results showed that the CV of electrical conductivity, GPP, GP, NSP, SISP, IBSP et al. was more than 50%. 100 seeds weight had significant negative correlation with GPP, GP and NSP, and significant positive correlation with electrical conductivity. Two principal components ?healthy factor? and ?transition factor? were extracted, with contribution ratio 64.45% and 15.83% respectively. Based on two contribution ratio matrix, 137 soybean materials were clustered into 3 types. And 5 types of these materials were clustered by comprehensive index method，9 soybean materials showed high seed-flooding tolerance. 5 soybean materials showed both high seed-flooding tolerance in comprehensive index and sample index method. 5 soybean materials could be used in seed-flooding tolerance selection and identification work. Electrical conductivity of soaking liquid together with germination potential percentage could be used as the selection index in the soybean mass seed- flooding tolerance selection. |
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| Keywords: | soybean seed-flooding tolerance waterlogging germplasm |
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