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东方白鹳微卫星遗传标记的筛选及其在种群遗传分析中的应用
引用本文:黄赟,周立志.东方白鹳微卫星遗传标记的筛选及其在种群遗传分析中的应用[J].中国鸟类,2011,2(1):33-38.
作者姓名:黄赟  周立志
作者单位:安徽大学资源与环境工程学院,安徽大学生物多样性与湿地生态研究所,合肥,230601
基金项目:This,research,was,supported,by,the,National,Natural,Science,Foundation,of,China,the,Anhui,Academic,and,Technical,Reserve,Candidate,Leaders,Fund
摘    要:东方白鹳(Ciconia boyciana)是分布于东亚的大型濒危涉禽,亟待深入开展其种群的保护遗传学研究.本研究采用微卫星标记技术筛选东方白鹤的微卫星位点,并进行种群的遗传多样性分析.所用的36对微卫星引物7对来自白鹳(Ciconia ciconia),12对来自朱鹮(Nipponia nippon),17对来自大蓝...

关 键 词:微卫星分子标记  东方白鹳(Ciconia  boyciana)  遗传多样性

Screening and application of microsatellite markers for genetic diversity analysis of Oriental White Stork (Ciconia boyciana)
Yun HUANG,Lizhi ZHOU.Screening and application of microsatellite markers for genetic diversity analysis of Oriental White Stork (Ciconia boyciana)[J].Chinese Birds,2011,2(1):33-38.
Authors:Yun HUANG  Lizhi ZHOU
Abstract:The Oriental White Stork (Ciconia boyciana) is a large and endangered waterbird in East Asia.Research on conservation genetics of this species is urgently needed. In this study, microsatellite marking technology was used for screening and analysis of genetic diversity of microsatellite markers in Oriental White Storks. A total of 36 pairs of microsatellite primers were used, of which 7 pairs came from Ciconia ciconia, 12 pairs from Nipponia nippon and 17 pairs from Ardea herodias. Microsatellite loci were screened from 23 individuals of the Oriental White Stork and 11 microsatellite loci were found with high polymorphism. The number of population alleles ranged from 3 to 11, averaging 7.09. The average expected heterozygosity (He) was 0.7816 and the average polymorphism information content (PIG) 0.7172, suggesting a relatively high genetic diversity in the population.
Keywords:microsatellite molecular marker  Ciconia boyciana  genetic diversity
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