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| 应用间接血凝试验(IHA)诊断猪蛔虫病 | |
| 引用本文： | 何光志,田维毅,简昌友,刘素,安永如.应用间接血凝试验(IHA)诊断猪蛔虫病[J].贵州农业科学,2010,38(8). |
| 作者姓名： | 何光志 田维毅 简昌友 刘素 安永如 |
| 作者单位： | 1. 贵阳中医学院,贵州,贵阳550002 2. 贵州省凤冈县人民医院,贵州,凤冈564200 3. 安顺市职业技术学院,贵州,安顺561000 |
| 基金项目： | 贵阳中医学院博士基金"似蚓蛔虫抗原基因(Al-Ag1)的克隆与序列分析" |
| 摘 要： | 以猪蛔虫抗原致敏绵羊红细胞制备检测猪蛔虫特异性抗体的间接血凝诊断试剂,与猪蛔虫抗体发生特异性凝集反应.结果表明,用间接血凝诊断试剂对3 000份猪血清进行现场检测,阳性率为30.00%;而琼脂扩散试验(AGP)的阳性率为13.00%,表明,间接血凝试验(IHA)比GAP敏感,间接血凝诊断试剂可用于猪蛔虫病的快速诊断. |
| 关 键 词： | 猪蛔虫 间接血凝试验(IHA) 诊断试剂 |
Application of Indirect Hemagglutination Assay(IHA) in Diagnosis of Ascariasis |
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| HE Guang-zhi,TIAN Wei-yi,JIAN Chang-you,LIU Su,AN Yong-ru.Application of Indirect Hemagglutination Assay(IHA) in Diagnosis of Ascariasis[J].Guizhou Agricultural Sciences,2010,38(8). | |
| Authors: | HE Guang-zhi TIAN Wei-yi JIAN Chang-you LIU Su AN Yong-ru |
| Institution: | HE Guang-zhi1,TIAN Wei-yi1,JIAN Chang-you2,LIU Su2,AN Yong-ru3(1.Guiyang College of Traditional Chinese Medicine,Guiyang,Guizhou 550002,2.Fenggang People's Hospital,Fenggang,Guizhou 564200,3.Anshun Vocational and Technical College,Anshun,Guizhou 561000,China) |
| Abstract: | The indirect hemagglutination diagnostic reagent with specific antibody of Ascaris suum was produced from sheep erythrocytes sensitized with the antigen from Ascaris suum.3 000 pig serum samples were detected by the indirect hemagglutination diagnostic reagent with the specific antibody of Ascaris suum on-the-spot.The results showed that the positive rate of IHA and AGP reached 30.00% and 13.00% respectively,which indicates that the indirect hemagglutination assay,which is more sensitive than GAP,can be use... |
| Keywords: | Ascaris suum indirect hemagglutination assay(IHA) diagnostic reagent |
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