| 猪Annexin A2基因真核表达载体的构建及表达 |
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| 引用本文： | 邢士博,曹宇航,张田,朱琳,陈玉珏,任林柱.猪Annexin A2基因真核表达载体的构建及表达[J].吉林农业大学学报,2012,34(5):571-574. |
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| 作者姓名： | 邢士博 曹宇航 张田 朱琳 陈玉珏 任林柱 |
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| 作者单位： | 吉林大学畜牧兽医学院,长春,130062 |
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| 基金项目： | 2011年吉林大学基本科研业务费资助项目;2011年吉林大学“大学生创新实验计划”校一级项目(2011B81214) |
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| 摘 要： | 根据猪的Annexin A2基因序列设计引物,通过PCR方法扩增Annexin A2基因cDNA,并将其克隆到pGEM-T载体后进行测序分析,然后将测序结果正确的Annexin A2基因cDNA亚克隆到pEGFP-N1表达载体上,成功构建了表达载体.将表达载体瞬时转染Marc-145细胞,结果表明:表达载体pEGFP-N1 -ANXA2可以在Marc-145细胞中表达.
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| 关 键 词： | Annexin A2 基因 猪 真核表达 |
Construction and Expression of Eukaryotic Expression Vector of Porcine Annexin A2 Gene |
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| XING Shi-bo,CAO Yu-hang,ZHANG Tian,ZHU Lin,CHEN Yu-jue,REN Lin-zhu.Construction and Expression of Eukaryotic Expression Vector of Porcine Annexin A2 Gene[J].Journal of Jilin Agricultural University,2012,34(5):571-574. |
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| Authors: | XING Shi-bo CAO Yu-hang ZHANG Tian ZHU Lin CHEN Yu-jue REN Lin-zhu |
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| Institution: | College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China |
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| Abstract: | cDNA of Annexin A2 gene was amplified using porcine mRNA as template by RT-PCR,and cloned into pGEM-T vector.Then,the cDNA was subcloned into pEGFP-N1 to construct eukaryotic expression vector pEGFP-N1-ANXA2 of Annexin A2.The results of transfection showed that the eukaryotic expression vector pEGFP-N1-ANXA2 of Annexin A2 was constructed successfully.The vector can be expressed in Marc-145 cells.The result in present study will be useful in studying PRRSV infection. |
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| Keywords: | Annexin A2 gene porcine eukaryotic expression |
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