| 桂花离体培养与快速繁殖技术的初步研究 |
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| 引用本文： | 宋会访,葛红,周媛,王彩云.桂花离体培养与快速繁殖技术的初步研究[J].园艺学报,2005,32(4):738-742. |
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| 作者姓名： | 宋会访 葛红 周媛 王彩云 |
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| 作者单位： | 华中农业大学园艺林学学院, 武汉430070; 中国农业科学院蔬菜花卉研究所, 北京100081 |
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| 基金项目： | 国家‘863’计划项目(2001AA241201) |
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| 摘 要： | 以桂花(Osmanthus fragrans Lour. ) 胚和新梢茎段为外植体进行了离体培养与快速繁殖研究,筛选出最适培养基——— (1) 胚萌发: MS +BA 1.00 mg·L - 1 +蔗糖3%; ( 2) 新梢茎段启动培养: LMc +KT 8.00 mg·L - 1+NAA 0.10 mg·L - 1 +蔗糖3%; ( 3) 继代增殖培养: LMc + TDZ 0.50 mg·L - 1 +NAA0.10 mg·L - 1 +蔗糖3%; (4) 生根培养: 1/2MS +NAA 2.00 mg·L - 1 +蔗糖3%。采用腐殖质土为栽培基质, 移栽成活率可达80%以上。
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| 关 键 词： | 桂花 离体培养 快速繁殖 |
| 文章编号： | 0513-353X（2005）04-0738-03 |
| 收稿时间： | 2004-09-28 |
| 修稿时间： | 2004-09-282004-11-15 |
Primary Study on in Vitro Culture and Micropropagation of Sweet Osmanthus |
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| Song Huifang,Ge Hong,Zhou Yuan,Wang Caiyun.Primary Study on in Vitro Culture and Micropropagation of Sweet Osmanthus[J].Acta Horticulturae Sinica,2005,32(4):738-742. |
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| Authors: | Song Huifang Ge Hong Zhou Yuan Wang Caiyun |
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| Institution: | 1.Department of Medical Image,2Department of Pathology, The First Affiliated Hospital of Jinan University, Guangzhou 510632, China |
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| Abstract: | Using the embryo, stem-segment of new shoots as the exp lants, in vitro culture and high frequency propagation of sweet osmanthus were studied. The results showed that the optimizing media for various stages were as follows: (1) Initial medium for embryo: MS +BA 1.00 mg·L - 1 + 3% sucrose; (2) Initialmedium of stem-segment for new shoots: LMc + KT 8.00 mg·L - 1 +NAA 0.10 mg·L - 1 + 3% sucrose; (3) Clump shoot regeneration medium: LMc + TDZ 0.50 mg·L - 1 +NAA 0.10 mg·L - 1 + 3% sucrose; ( 4) Rooting medium: 1 /2MS +NAA 2.00 mg·L - 1 + 3% sucrose. Using humus soil as culture substrate, thesurvival percentage of plantlets could reach 80%. |
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| Keywords: | Sweet osmanthus In vitro culture Rapid propagation |
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