| 金黄色葡萄球菌蛋白A单克隆抗体的制备 |
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| 引用本文： | 彭洪江,杜惠芬,李克生,马卫静,杜丽娟,袁明,连晓雯.金黄色葡萄球菌蛋白A单克隆抗体的制备[J].甘肃农业大学学报,2012,47(6):1-5,12. |
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| 作者姓名： | 彭洪江 杜惠芬 李克生 马卫静 杜丽娟 袁明 连晓雯 |
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| 作者单位： | 1. 甘肃农业大学动物医学院,甘肃兰州730070;甘肃省医学科学研究院医学生物中心,甘肃兰州730050
2. 甘肃省医学科学研究院医学生物中心,甘肃兰州,730050 |
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| 基金项目： | 甘肃省科教兴省省长基金 |
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| 摘 要： | 为获得稳定性好、特异性强、效价较高的抗金黄色葡萄球菌蛋白A单克隆抗体,用热灭活的金黄色葡萄球菌wol-04菌体抗原免疫BALB/c小鼠,取免疫小鼠脾细胞与小鼠骨髓瘤细胞(SP2/0)融合,以金黄色葡萄球菌蛋白A(SPA)为筛选抗原进行克隆筛选,并对各株单抗的生物学特性进行分析和鉴定.结果表明:试验获得了3株稳定分泌SPA单克隆抗体的杂交瘤细胞,其中单克隆抗体3B11、4F7为SPA表面抗原表位的单克隆抗体,与金黄色葡萄球菌wol-04菌体和SPA有较强的免疫反应性,免疫球蛋白亚类分别为IgG1和IgG3,具有稳定性好、特异性强、效价较高的特点.
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| 关 键 词： | 金黄色葡萄球菌蛋白A 单克隆抗体 杂交瘤细胞 间接ELISA |
Monoclonal antibodies production against Staphylococcus aureus protein A |
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| PENG Hong-jiang
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DU Hui-fen
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LI Ke-sheng
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MA Wei-jing
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DU Li-juan
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YUAN Ming
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LIAN Xiao-wen.Monoclonal antibodies production against Staphylococcus aureus protein A[J].Journal of Gansu Agricultural University,2012,47(6):1-5,12. |
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| Authors: | PENG Hong-jiang DU Hui-fen LI Ke-sheng MA Wei-jing DU Li-juan YUAN Ming LIAN Xiao-wen |
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| Institution: | 1.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;2.Center of Medical Biotechnology,Gansu Academy of Medical Sciences,Lanzhou 730050,China) |
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| Abstract: | In order to produce monoclonal antibodies(McAbs) against Staphylococcus aureus protein A(SPA),hybridoma cells were obtained by fusion of myeloma cells with lymphocytes from BALB/c mice immunized with heat-killed S.aureus cells.Positive clones were selected using indirect ELISA with S.aureus and SPA as antigens.The results showed that six McAbs specific for SPA of S.aureus were obtained.Two of McAbs,3B11 and 4F7 were proved to be specific and agglutinated S.aureus wol-04 cells well.They were IgG1 and IgG3 subtype,with high specificity,stability and titer. |
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| Keywords: | Staphylococcus aureus protein A monoclonal antibodies hybridoma cells indirect ELISA |
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