| 福建黄牛的遗传多态性及分类 |
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| 引用本文： | 刘庆华,甘乾福,梁学武.福建黄牛的遗传多态性及分类[J].福建农林大学学报(自然科学版),2007,36(6):604-607. |
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| 作者姓名： | 刘庆华 甘乾福 梁学武 |
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| 作者单位： | 福建农林大学动物科学学院,福建,福州,350002 |
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| 基金项目： | 福建省科技厅重大科技项目 |
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| 摘 要： | 用8个微卫星标记对福建黄牛的等位基因频率、多态信息含量(PIC)、遗传杂合度(H)和有效等位基因数(E)等指标进行统计分析,并在此基础上对其进行聚类分析和分类研究.结果表明:(1)8个微卫星标记在所检测的福建黄牛群体中都表现出较丰富的多态性,4个群体8个微卫星座位共有58个等位基因,每个微卫星标记平均检测到7.2个等位基因(3-11);8个微卫星标记的PIC平均为0.6471(0.3942-0.8062),各群体的PIC差异不显著;全部群体的H为0.2930;各群体的E平均为3.37(3.03-3.84).这显示黄牛群体的等位基因频率、PIC、H和E等指标有较好的一致性,说明福建黄牛品种内在微卫星位点上均具有丰富的遗传多样性.(2)根据Ne i氏标准遗传距离进行UPGMA聚类分析的结果显示,4个群体间的遗传变异不大.福建黄牛地方品种在微卫星位点上具有丰富的遗传多样性;遗传距离及聚类分析结果表明,这些群体黄牛属于同一个地方品种.
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| 关 键 词： | 福建黄牛 微卫星标记 遗传多样性 |
| 文章编号： | 1671-5470(2007)06-0604-04 |
| 修稿时间： | 2006年5月10日 |
Genetic diversity and classification of Fujian yellow cattle |
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| LIU Qing-hua,GAN Qian-fu,LIANG Xue-wu.Genetic diversity and classification of Fujian yellow cattle[J].Journal of Fujian Agricultural and Forestry University,2007,36(6):604-607. |
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| Authors: | LIU Qing-hua GAN Qian-fu LIANG Xue-wu |
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| Abstract: | The genetic diversity of eight microsatellite sites was studied by PCR,and the allele frequency,polymorphism information content(PIC),heterozygosity(H) and effective number of alleles(E) in different cattle populations were analyzed with statistics method.At the same time,the cluster of these cattle has also been studied.The results were showed as follow.(1) The eight microsatellite sites analyzed were polymorphic in these cattle populations.The average numbers of alleles and PIC in each site were 7.2(3-11) and 0.6471(0.3942-0.8062) respectively.The difference of average PIC among these populations was not significant.The average H in all populations was 0.2930,the average E was 3.37 (3.03-3.84).These results indicated that there was a good compatibility between the parameters including allele frequencies,PIC,H and E in different cattle populations.It also showed that they were rich in genetic polymorphisms of microsatellite sites within a breed and between breeds of the cattle.(2) According to Neis standard genetic distance,the UPGMA dendrogram indicated that there was little genetic difference among four populations.They were rich in genetic polymorphisms of microsatellite sites within a breed of Minnan yellow cattle.This cluster result showed that these populations belong to the same breed. |
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| Keywords: | Fujian yellow cattle microsatellite DNA genetic diversity |
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