| H7亚型禽流感病毒HA1基因在重组杆状病毒中的表达及其表达产物的抗原性 |
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| 引用本文： | 贾珊珊,孙凌霜,齐岩,孙进华,姜亦飞,王君伟.H7亚型禽流感病毒HA1基因在重组杆状病毒中的表达及其表达产物的抗原性[J].中国兽医科技,2007,37(3):232-235. |
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| 作者姓名： | 贾珊珊 孙凌霜 齐岩 孙进华 姜亦飞 王君伟 |
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| 作者单位： | 东北农业大学动物医学院,黑龙江哈尔滨150030 |
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| 基金项目： | 国家科技攻关计划项目（2004BA519A23） |
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| 摘 要： | 从pMD18-T—HA阳性质粒中扩增出H7亚型禽流感病毒（AIV）HA1基因，并亚克隆至昆虫杆状病毒转移载体pBlueBacHis2A，将筛选的重组质粒命名为pBlueBacHisH7-HA1，与线性化杆状病毒DNA（Bac—N—BlueDNA）共转染sf9昆虫细胞，挑取蓝色蚀斑，经3次蚀斑纯化，得到重组杆状病毒rpBlue-HisH7HA1。Western-blotting和间接免疫荧光试验结果显示，HA1在昆虫细胞中得到表达，且表达产物具有抗原性。抗原特异性试验证明，重组HA1蛋白与鸡H5、H9亚型AIV抗血清不发生交叉反应。血凝试验证明，重组HA1蛋白具有良好的血凝性。
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| 关 键 词： | 禽流感病毒 HA1基因 重组杆状病毒 H7亚型 |
| 文章编号： | 1673-4696（2007）03-0232-04 |
| 收稿时间： | 2006-10-31 |
| 修稿时间： | 2007-02-25 |
Expression of HA1 gene of avian influenza virus H7 subtype in recombinant baculovirus and analysis of antigenicity of the expressed HA1 protein |
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| JIA Shan-shan, SUN Ling-shuang, QI Yan, SUN Jin-hua,JIANG Yi-fei, WANG Jun-wei.Expression of HA1 gene of avian influenza virus H7 subtype in recombinant baculovirus and analysis of antigenicity of the expressed HA1 protein[J].Chinese Journal of Veterinary Science and Technology,2007,37(3):232-235. |
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| Authors: | JIA Shan-shan SUN Ling-shuang QI Yan SUN Jin-hua JIANG Yi-fei WANG Jun-wei |
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| Abstract: | The HA1 gene of avian influenza virus H7 subtype was amplified from recombinant plasmid pMD-18-T-HA by PCR. The PCR product was subcloned into pBlueBacHis2A vector. The recombinant transfer vector pBlueHisH7-HA1 was screened at random. After pBlueHisH7-HA1 was co-transfected with Bac-N-Blue DNA by cationic liposome-mediated transfection, and was purified by three cycles of plaque assay with chromogenic substracte X-gal in low temperature-melting agarose, a recombinant baculovirus, designated as rpBlueHisH7-HAl,was obtained. Western-blotting and indirect immunofluorescence tests showed the HA1 gene was well expressed,and the expressed product had antigenicity. An antigen specific test showed that the recombinant did not have any cross-reaction with chicken H5 and H9 antisera. A hemagglutinin test certified that it had good hemagglutination. |
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| Keywords: | avian influenza virus HA1 gene recombinant baculovirus H7 subtype |
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