A technique of screening tuber-bearing Solanums for resistance to the root knot nematode |
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| Authors: | K K Nirula Pushkarnath |
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| Institution: | (1) Central Potato Research Institute, Simla, India |
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| Abstract: | A simple method for screening commercial varieties of potato and other wild and cultivated tuber-bearing Solanums for resistance to root knot nematode Meloidogyne incognita (Kofoid and White) Chitwood, has been described. Leaflets taken from plants which were 6 to 8 weeks old are treated with 5 ppm IBA for 24 hours. Treated leaflets, when kept in water, rooted in 12 to 15 days. Use of paper cups, each containing 50 gm of autoclaved soil, was found to be adequate for free development of the roots from the petiole region of the leaflets. Inoculating the soil in the cups with 20 larvae and incubating them at 25°C for 10 days was found to create good conditions for infection of rootlets and subsequent gall formation by the nematodes. The degree of gall formation on the roots was recorded as the grade of susceptibility or resistance of the plant material under test. |
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