| 四种方法对鲢血清免疫球蛋白的纯化及分子量测定 |
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| 引用本文： | 孙军,高谦,聂品.四种方法对鲢血清免疫球蛋白的纯化及分子量测定[J].浙江大学学报(农业与生命科学版),2005,31(4):487-492. |
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| 作者姓名： | 孙军 高谦 聂品 |
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| 作者单位： | 1. 中国科学院水生生物研究所,淡水生态与生物技术国家重点实验室,湖北,武汉,430072;暨南大学水生生物研究所,广东,广州,510632
2. 中国科学院水生生物研究所,淡水生态与生物技术国家重点实验室,湖北,武汉,430072 |
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| 摘 要： | 采用mannan-binding protein(MBP)、TG及免疫亲和层析法和饱和硫酸铵沉淀法纯化鲢血清免疫球蛋白IgM,并比较了4种纯化方法的纯化效果.SDS-PAGE显示鲢血清免疫球蛋白的重链和轻链分子量分别为76.4 kD和27.2 kD,推算其总分子量约828.8kD
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| 关 键 词： | 鲢 血清 免疫球蛋白 纯化 |
| 文章编号： | 1008-9209(2005)04-0487-06 |
| 修稿时间： | 2004年9月6日 |
Isolation, purification and molecular weight determination of serum immunoglobulin from silver carp Hypophthalmichthys molitrix |
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| SUN Jun,GAO Qian,NIE Pin.Isolation, purification and molecular weight determination of serum immunoglobulin from silver carp Hypophthalmichthys molitrix[J].Journal of Zhejiang University(Agriculture & Life Sciences),2005,31(4):487-492. |
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| Authors: | SUN Jun GAO Qian NIE Pin |
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| Abstract: | Silver carp serum immunoglobulin was purified using immunoaffinity chromatographic column, and the serum IgM with high purity was then obtained. The IgM H chain and L chain were estimated to be about 76.4 kD and 27.2 kD, respectively. Simultaneously, mannan-binding protein (MBP) affinity chromatography, saturated ammonium sulfate (SAS) sedimentation and TG affinity chromatography methods were also used to purify the serum IgM. Although serum IgM was also successfully isolated by these methods, the purity attained differed, however, from that attained when the previous method was employed. It is considered that immunoaffinity method is more desirable in purification of fish serum immunoglobulin. |
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| Keywords: | silver carp serum immunoglobulin purification |
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