| 镉/铬胁迫对谷子幼苗生长和NADPH氧化酶及抗氧化酶体系的影响 |
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| 引用本文： | 田保华,张彦洁,张丽萍,马晓丽,金竹萍,刘志强,刘旦梅,裴雁曦.镉/铬胁迫对谷子幼苗生长和NADPH氧化酶及抗氧化酶体系的影响[J].农业环境科学学报,2016,35(2):240-246. |
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| 作者姓名： | 田保华 张彦洁 张丽萍 马晓丽 金竹萍 刘志强 刘旦梅 裴雁曦 |
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| 作者单位： | 山西大学生命科学学院,太原,030006 |
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| 基金项目： | 山西省青年科技研究基金(2014021026-2);山西省青年科技研究基金(2014021026-1);国家青年基金项目(31501772) |
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| 摘 要： | 为了探讨重金属镉(Cd)、铬(Cr)胁迫对谷子幼苗生长和生理的影响,采用营养液培养法,检测不同浓度的Cd、Cr胁迫对谷子幼苗生长以及过氧化氢(H_2O_2)含量、叶绿素含量、丙二醛(MDA)含量、NADPH氧化酶和抗氧化酶活性的影响。结果表明:不同浓度(50/1000μmol·L~(-1))两种金属(Cd、Cr)胁迫下,谷子幼苗株高、根长显著减小(P0.05),H_2O_2和MDA含量升高,叶绿素含量降低;随Cd、Cr的浓度升高,根和叶片中NADPH氧化酶活性显著增强,高浓度Cd、Cr可导致过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性显著升高;同时Cd、Cr诱导编码NADPH氧化酶基因Sirboh D和Sirboh F的表达,而超氧化物歧化酶(SOD)活性呈低浓度增加高浓度下降的趋势。由此可知,Cd、Cr对谷子幼苗的胁迫使其发生一系列生理指标变化,造成植株不同程度损伤,植株自身则通过增强抗氧化酶活性对机体起到保护作用。
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| 关 键 词： | 镉 铬 NADPH氧化酶 谷子 |
| 收稿时间： | 2015/9/11 0:00:00 |
Effects of cadmium or chromium on growth and NADPH oxidase and antioxidant enzyme system of foxtail millet seedlings |
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| TIAN Bao-hu,ZHANG Yan-jie,ZHANG Li-ping,MA Xiao-li,JIN Zhu-ping,LIU Zhi-qiang,LIU Dan-mei and PEI Yan-xi.Effects of cadmium or chromium on growth and NADPH oxidase and antioxidant enzyme system of foxtail millet seedlings[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2016,35(2):240-246. |
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| Authors: | TIAN Bao-hu ZHANG Yan-jie ZHANG Li-ping MA Xiao-li JIN Zhu-ping LIU Zhi-qiang LIU Dan-mei and PEI Yan-xi |
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| Institution: | School of Life Science, Shanxi University, Taiyuan 030006, China,School of Life Science, Shanxi University, Taiyuan 030006, China,School of Life Science, Shanxi University, Taiyuan 030006, China,School of Life Science, Shanxi University, Taiyuan 030006, China,School of Life Science, Shanxi University, Taiyuan 030006, China,School of Life Science, Shanxi University, Taiyuan 030006, China,School of Life Science, Shanxi University, Taiyuan 030006, China and School of Life Science, Shanxi University, Taiyuan 030006, China |
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| Abstract: | Different heavy metals can induce various stress tolerance mechanisms in plants. Here a solution culture was used to investigate the effects of different concentrations of cadmium(Cd) or chromium(Cr) on growth, hydrogen peroxide(H2O2), chlorophyll, malondialdehyde(MDA) concentration, NADPH oxidase and antioxidant enzyme activities of foxtail millet seedlings. Results showed that plant height, root length and chlorophyll content were inhibited by Cd or Cr. Content of H2O2 and MDA was increased under Cd or Cr stresses. Increasing Cd or Cr concentrations increased NADPH oxidase activity in roots and leaves by inducing the expression of related genes, Sirboh D and Sirboh F, and peroxidase(POD), catalase(CAT) and ascorbate peroxidase(APX) activities at high concentrations. Superoxide dismutase(SOD) activity increased at low Cd or Cr concentrations, whereas it decreased at high concentrations. These results indicate that heavy metal stresses in foxtail millet alter physiological parameters, and cause various plant damages, but may also increase antioxidant enzyme activities, thus protecting the plant from further damages. |
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| Keywords: | cadmium chromium NADPH oxidase foxtail millet |
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