| 新高系梨10个品种S基因型的鉴定 |
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| 引用本文： | 袁德义,谭晓风,张琳,赵思东,乌云塔娜,段经华,曹玉芬.新高系梨10个品种S基因型的鉴定[J].园艺学报,2007,34(6):1353-1360. |
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| 作者姓名： | 袁德义 谭晓风 张琳 赵思东 乌云塔娜 段经华 曹玉芬 |
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| 作者单位： | (中南林业科技大学经济林育种与栽培国家林业局重点实验室，长沙 410004；中国农业科学院果树研究所，兴城 125100) |
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| 基金项目： | 林业部科研项目;引进国际先进农业科技计划(948计划) |
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| 摘 要： | 以亲缘关系极近的新高系梨10个品种及被视为‘新高’亲本的‘天之川’和‘今村秋’梨等为试验材料，对其基因组DNA进行了PCR-RFLP检测、DNA序列测定及生物信息学分析。结果表明：新高系梨10个品种即‘新高’、‘黄金’、‘水晶’、‘早生黄金’、‘早蜜新高’、‘天皇’、‘金秋’、‘晚大新高’、‘农家新高’和‘鲜黄’的S基因型分别为S3S9、S3S4、S3S9、S3S4、S3S9、S3S9、S3S9、S3S9、S3S9和S3S5；‘天之川’、‘今村秋’的S基因型分别为S1S9和S1S6，通过基因型判定‘今村秋’非‘新高’梨的父本。
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| 关 键 词： | 梨 砂梨 新高系梨 自交不亲和性 S基因型 鉴定 |
| 文章编号： | 0513-353X（2007）06-1353-08 |
| 收稿时间： | 2007-05-07 |
| 修稿时间： | 2007-09-30 |
Identification of S-genotype of Ten Cultivars from Niitaka Line Pear |
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| YUAN De-yi,TAN Xiao-feng,ZHANG Lin,ZHAO Si-dong,WUYUN Ta-na,DUAN Jing-hua,CAO Yu-fen.Identification of S-genotype of Ten Cultivars from Niitaka Line Pear[J].Acta Horticulturae Sinica,2007,34(6):1353-1360. |
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| Authors: | YUAN De-yi TAN Xiao-feng ZHANG Lin ZHAO Si-dong WUYUN Ta-na DUAN Jing-hua CAO Yu-fen |
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| Institution: | (;1.The Key Laboratory of Non-wood Forest Product of Forestry Ministry, Central-South of Forestry and Technology University, Changsha 410004, China;2. Fruit Tree Research Institute，Chinese Academy of Agricultural Sciences，Xingcheng 125100，China) |
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| Abstract: | Ten cultivars with extremely close relationship from Niitaka line pear were used as materials for S-genotyping. In addition, two cultivars 'Imamuraaki' and 'Amanogawa' that were considered to be the parents of cultivar 'Niitaka' were also included. PCR-RFLP analysis of genomic DNAs of the 12 cultivars, nucleotide sequencing of amplified fragments and sequence analysis were performed. As the results, the ten cultivars of Niitaka line were genotyped as follows: 'Niitaka' (S3S9), 'WhangKeumBae' (S3S4), 'Sujeong' (S3S9), 'Josengwhangkeum'(S4S9), 'Soumituniitaka'(S3S9), 'Yewang'(S3S9), 'Jinqiu'(S3S9), 'Banndainiitaka' (S3S9), 'Nokaniitaka'(S3S9), and 'Sunhwang' (S3S5). The S-genotype of 'Amanogawa' and 'Imamuraaki'was identified as S1S9 and S1S6, respectively, suggesting that 'Imamuraaki'is unlikely to be the pollen parent of 'Niitaka'. |
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| Keywords: | Pear Pyrus pyrifolia Niitaka line pear Self-incompatibility S-genotype Identification |
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