| 表现腺胃炎的蛋用型鸡J亚群-白血病病毒的分离与鉴定 |
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| 引用本文： | 孙淑红,柴家前,王波,孙洪磊,王晓云,崔治中.表现腺胃炎的蛋用型鸡J亚群-白血病病毒的分离与鉴定[J].畜牧兽医学报,2010,41(2). |
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| 作者姓名： | 孙淑红 柴家前 王波 孙洪磊 王晓云 崔治中 |
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| 作者单位： | 山东农业大学,动物科技学院/动物医学院,泰安,271018 |
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| 基金项目： | 山东省中青年科学家基金，山东省农业重大应用技术创新课题，山东农业大学青年科技创新基金 |
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| 摘 要： | 从表现腺胃炎的尼克珊瑚粉商品代蛋鸡中分离到J亚群-白血病病毒(ALV-J)。将病料或鸡白细胞接种于CEF,培养12 d,分别采用单克隆抗体间接免疫荧光试验检测,结果10只鸡中有9只鸡分离到ALV-J,其中有4只鸡还存在与禽网状内皮增生病病毒(REV)的共感染。通过PCR扩增gp85基因,与已发表的20株ALV-J进行同源性比较。结果表明,与来自白羽肉鸡的HPRS103的同源性为97.8%,而与来自蛋用型鸡的SD07LK1株的同源性为93.0%。本研究发现,在某些仅仅发生腺胃炎的鸡也可能普遍存在ALV-J感染,再次显示了腺胃炎病料中病毒感染的多样性。ALV-J可能成为致腺胃炎的病原之一,但其致病作用有待进一步研究。
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| 关 键 词： | 腺胃炎 蛋用型鸡 J亚群-禽白血病病毒 |
Subgroup J Avian Leukosis Viruses Isolated from Egg-type Chickens with Proventriculitis |
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| SUN Shu-hong,CHAI Jia-qian,WANG Bo,SUN Hong-lei,WANG Xiao-yun,CUI Zhi-zhong.Subgroup J Avian Leukosis Viruses Isolated from Egg-type Chickens with Proventriculitis[J].Acta Veterinaria et Zootechnica Sinica,2010,41(2). |
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| Authors: | SUN Shu-hong CHAI Jia-qian WANG Bo SUN Hong-lei WANG Xiao-yun CUI Zhi-zhong |
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| Institution: | SUN Shu-hong,CHAI Jia-qian,WANG Bo,SUN Hong-lei,WANG Xiao-yun,CUI Zhi-zhong(College of Animal Science , College of Veterinary Medicine,Sh,ong Agricultural University,Taian 271018,China) |
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| Abstract: | Subgroup J avian leukosis virus(ALV-J) was isolated from both provetriculus suspensions and blood samples of 9 in 10 laying hens only with proventriculitis,and co-infection of ALV-J with reticuloendotheliosis virus was also confirmed in 4 chickens.The gp85 genes of ALV-J isolates were amplified by PCR,sequenced and compared to 20 reference ALV-J strains.Sequence comparisons indicated that the provetriculus isolate had 97.8% identity with the HPPS103 strain isolated from white meat-type chickens and 93.0% id... |
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| Keywords: | proventriculitis egg-type chickens subgroup J avian leukosis viruses |
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