| 应用琼脂免疫扩散试验检测猪伪狂犬病抗体的研究 |
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| 引用本文： | 孙刚,杜宗沛,高忠信,姜玉娴,颜世波,孙建,蒋淑英.应用琼脂免疫扩散试验检测猪伪狂犬病抗体的研究[J].黑龙江畜牧兽医,2000(4):8-9. |
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| 作者姓名： | 孙刚 杜宗沛 高忠信 姜玉娴 颜世波 孙建 蒋淑英 |
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| 作者单位： | [1]黑龙江省兽医卫生防疫站,黑龙江哈尔滨 [2]汤原县畜牧局,黑龙江汤原 |
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| 摘 要： | 建立了ＡＧＩＤ用于猪伪狂犬病（Ｐｓｅｕｄｏｒａｂｉｅｓ，Ｐｒ）的诊断。对９８份被俭血清的ＡＧＩＤ结果与ＳＮ结果比较，当ＳＮ滴度大于１：８时两者的阳性检出符合率为１００％，ＳＮ滴度小于或等于１：８时，两者的阳性检出符合率为８７．５％，ＡＧＩＤ和ＳＮ总的阳性符合率为９４．４％，结果表明，ＡＧＩＤ对Ｐｒ可进行特异性诊断，流行病学调查和免疫动态监测。
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| 关 键 词： | 猪伪狂犬病 琼脂免疫扩散试验 抗体检测 |
| 文章编号： | 1004-7034(2000)04-0008-02 |
| 修稿时间： | 2000年1月10日 |
Studies on Detecting Antibodies of Swine Pseudorabies with AGID |
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| SUN Gang,DU Zong-pei,GAO Zhong-xin,et al.Studies on Detecting Antibodies of Swine Pseudorabies with AGID[J].Heilongjiang Animal Science And veterinary Medicine,2000(4):8-9. |
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| Authors: | SUN Gang DU Zong-pei GAO Zhong-xin |
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| Abstract: | In this paper,the agar immunodiffusion test (AGID)for the detection of pseudorabies virus(PrV)antibodies in swine sera were developed.The sensitivity of the AGID was compared with the serum-neutralization(SN)test by 98 swine sera for PrV antibodies.Sera that had SN titers above were all positive by the AGID 24 serum samples with SN titers below 1:8,21 were A GID Positive,a 87.5% correlation.The overall sensivity of the A GID was 94.4%.These results suggested that the AGID is specifitic test,which is adapted to provide a method for immune detection and epidemiological investigation. |
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| Keywords: | pseudorabies agar immunodiffusion test serum neutralization test |
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