| 柱前衍生HPLC法检测罗非鱼肌肉中的硫酸新霉素 |
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| 引用本文： | 宋洁,王伟利,姜兰,王贤玉.柱前衍生HPLC法检测罗非鱼肌肉中的硫酸新霉素[J].广东农业科学,2011,38(3):142-143,147. |
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| 作者姓名： | 宋洁 王伟利 姜兰 王贤玉 |
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| 作者单位： | 1. 中国水产科学研究院珠江水产研究所,广东,广州,510380;上海海洋大学水产与生命学院,上海,201306
2. 中国水产科学研究院珠江水产研究所,广东,广州,510380 |
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| 基金项目： | 国家"十一五"科技支撑计划项目，广东省农业攻关项目 |
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| 摘 要： | 硫酸新霉素本身没有紫外吸收,必须通过柱前衍生来进行荧光榆测.建立合适的衍生方法是提高检测灵敏度的关键,通过对硫酸新霉素衍生方法的优化,确定衍生化学试剂氯甲酸戊甲酯乙腈溶液的浓度为8 mmol/L,存pH值为7.8的硼酸缓冲液中衍生反应15 min,10 h内检测可得到理想的结果.对硫酸新霉素检测方法优化后,该药物在罗非鱼肌肉中检测限可达到0.02mg/kg.定量限为0.05 mg/kg,在0.05～5 mg/kg浓度范围内有良好的线性关系(r2=0.9956),在肌肉中分别按照0.10、0.50、1.00 mg/kg的标准添加硫酸新霉素,回收率分别为76.43±1.46%、79.47±1.71%、86.82±1.13%.
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| 关 键 词： | 罗非鱼 硫酸新霉素 高效液相色谱法 氯甲酸芴甲酯 柱前衍生化 |
Determination of Neomycin sulfate in Tilapia muscle by high performance liquid chromatography with pre-column derivatization |
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| SONG Jie,WANG Wei-li,JIANG Lan,WANG Xian-yu.Determination of Neomycin sulfate in Tilapia muscle by high performance liquid chromatography with pre-column derivatization[J].Guangdong Agricultural Sciences,2011,38(3):142-143,147. |
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| Authors: | SONG Jie WANG Wei-li JIANG Lan WANG Xian-yu |
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| Institution: | SONG Jie1,2,WANG Wei-li1,JIANG Lan1,WANG Xian-yu1(1.Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510380,China,2.College of Fishery and Life Science,Shanghai Ocean University,Shanghai 201306,China) |
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| Abstract: | Neomycin sulfate has no UV absorption and the fluorescence detection have to use pre-column derivatization.Establish a appropriate derivatization method is crucial to improve the detection sensitivity.Optimal conditions for the derivatization of neomycin sulfate was described,the concentration of 9-fluorenylmethyl chloroformate in acetonitrile solution was 8 mmol/L,the reaction is 15 min in pH=7.8 of borate buffer,the derivative was detected in 10 h.The determination of Neomycin sulfate was optimized,the de... |
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| Keywords: | Tilapia neomycin sufate HPLC 9-fluorenylmethyl chloroformate pre-column derivatization |
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