| 中药植物金线吊乌龟对植物病原真菌的抗菌活性 |
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| 引用本文： | 玉艳珍,邓业成,张明,覃旭,李丽芬,骆海玉.中药植物金线吊乌龟对植物病原真菌的抗菌活性[J].植物保护,2010,36(2). |
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| 作者姓名： | 玉艳珍 邓业成 张明 覃旭 李丽芬 骆海玉 |
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| 作者单位： | 广西师范大学生命科学学院,药用资源化学与药物分子工程教育部重点实验室,桂林,541004 |
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| 基金项目： | 广西研究生教育创新计划项目，药用资源化学与药物分子工程教育部重点实验室资助课题，广西自然科学基金项目 |
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| 摘 要： | 采用菌丝生长速率法测定了中药植物金线吊乌龟块根甲醇提取物对5种植物病原真菌的抗菌活性,结果表明:金线吊乌龟块根提取物对梨锈病菌、稻瘟病菌、水稻纹枯病菌和玉米炭疽病菌有很高的抑制活性,质量浓度为10g/L时,抑菌率分别为97.74%、100.00%、94.51%和84.40%;有效中浓度(EC50)分别为0.5879、0.3122、0.2807g/L和0.8353g/L。对柿角斑病菌的活性较低,在10g/L质量浓度下,抑菌率只有67.59%,EC50为1.3491g/L。金线吊乌龟块根提取物对甘蔗凤梨病菌孢子、柑橘疮痂病菌孢子和梨锈病菌孢子的萌发具有明显的抑制活性,质量浓度为10g/L时,抑制率分别为91.80%、97.00%和100.00%;对西瓜枯萎病菌孢子萌发的抑制效果比较差,抑制率仅为20.63%。按生物碱分离程序对金线吊乌龟块根提取物进行初步分离,结合活性跟踪,发现氯仿层生物碱是金线吊乌龟的主要抗菌活性成分。
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| 关 键 词： | 金线吊乌龟 抗菌活性 活性物质 |
The antifungal activity of the Chinese herbal medicine Stephania cepharantha Hayata against plant pathogenic fungi |
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| Yu Yanzhen,Deng Yecheng,Zhang Ming,Qin Xu,Li Lifen,Luo Haiyu.The antifungal activity of the Chinese herbal medicine Stephania cepharantha Hayata against plant pathogenic fungi[J].Plant Protection,2010,36(2). |
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| Authors: | Yu Yanzhen Deng Yecheng Zhang Ming Qin Xu Li Lifen Luo Haiyu |
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| Abstract: | The antifungal activity of the methanol extract from the tuberous root of Stephania cepharantha Hayata against 5 kinds of plant pathogenic fungi was determined using mycelium growth rate method. The results showed that the extract from the tuberous root of S. Cepharantha had high inhibitory activity against Gymnosporangium haraeanum Syd., Pyricularia oryzae Cav., Rhizoctonia solani Kuhn, and Colletotrichum graminicola (Ces.) Wil-son., with the inhibition rates of 97.74%, 100.00%, 94.51% and 84.40% at 10 g/L, respectively, and the EC_(50) values of 0.587 9g/L, 0.312 2 g/L, 0.280 7 g/L and 0.835 3 g/L, respectively. The antifungal activity of the extract against Cercospora kaki Ell. Et Ev. Was weaker, with the inhibition rate of 67.59% at 10 g/L, and the EC_(50) value of 1.349 1 g/L. The extract of the tuberous root of S. Cepharantha also had significant inhibitory ac-tivity against spore germination of Thielaviopsis paradoxa (de Seynes) V. Hohnel, Sphaceloma fawcettii Jenkins and G. Haraeanum Syd., with the inhibition rates of 91.80%, 97.00% and 100.00% at 10 g/L, respectively. The inhibitory activity against spore germination of Fusarium oxysporum f. Sp. Niveum (E. F. Smith) Syn. Et Hans was weaker, with the inhibition rate of 20.63% at 10g/L. The methanol extract from the tuberous root of S. Cepharantha was preliminarily separated into 3 parts by the method of alkaloid isolation, and the results of bioas-say showed that the active ingredients mainly consisted in the chloroform fraction. |
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| Keywords: | Stephania cepharantha Hayata antifungal activity active fraction |
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