| 不同培养基对四倍体刺槐叶片不定芽诱导研究 |
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| 引用本文： | 燕丽萍,夏阳,梁慧敏,王太明,詹伟,柴传华.不同培养基对四倍体刺槐叶片不定芽诱导研究[J].山东林业科技,2007(1):23-24. |
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| 作者姓名： | 燕丽萍 夏阳 梁慧敏 王太明 詹伟 柴传华 |
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| 作者单位： | 1. 山东省林业科学研究院,济南,250014
2. 日照大沙洼林场 |
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| 摘 要： | 以四倍体刺槐不同着生节位大田叶片为试材,接种于3种不同培养基进行培养,诱导不定芽,使其形成再生植株。结果表明:以尚未充分展开复叶为最佳外植体,MS Salt B5Vitamin 1.8 mg/L NAA 1.1mg/L6-BA培养基为最佳诱导培养基,愈伤组织诱导率、芽分化率、苗增值系数最高,分别为96.7%、93.1%、9.1倍。
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| 关 键 词： | 四倍体刺槐 离体叶片 不定芽 |
| 文章编号： | 1002-2724（2007）01-0023-02 |
| 收稿时间： | 2006-11-15 |
| 修稿时间： | 2006年11月15 |
Study on the Shoot Regeneration of Teraploid Black Locust Leaf in Different Culture Mediums |
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| Yan Liping,et al..Study on the Shoot Regeneration of Teraploid Black Locust Leaf in Different Culture Mediums[J].Journal of Shandong Forestry Science and Technology,2007(1):23-24. |
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| Authors: | Yan Liping |
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| Institution: | Shandong Provincial Academy of Forestry, Shandong Jinan 250014 |
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| Abstract: | A research was conducted using leaves Teraploid Black Locust as explants in different culture mediums to establish a highly efficient regeneration system.The result indicated that The optimal culture medium is MSsalts B5 vitamins 1.8 mg/L NAA 1.1mg/L6-BA.callus inductivity,bud's differentiation rate,seedling's increment coefficient is the highest,96.7%,93.1%,9.1 times;the young tender 1st location of knots or nodes leaf for is the best explant,the bud differentiation percentage reaches as high as 90%,3rd,the 5th location of knots or nodes leaf bud differentiation percentage respectively is 43.3% and 0%. |
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| Keywords: | teraploid black locust leaves in vitro adventitious shoot |
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