| 镜鲤多家系体长和体质量QTL定位分析 |
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| 引用本文： | 郑先虎,匡友谊,吕伟华,曹顶臣,冬方,孙效文.镜鲤多家系体长和体质量QTL定位分析[J].水产学报,2014,38(9):1263-1269. |
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| 作者姓名： | 郑先虎 匡友谊 吕伟华 曹顶臣 冬方 孙效文 |
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| 作者单位： | 中国水产科学研究院黑龙江水产研究所, 淡水鱼类育种国家地方联合工程实验室, 黑龙江 哈尔滨 150070;中国水产科学研究院黑龙江水产研究所, 淡水鱼类育种国家地方联合工程实验室, 黑龙江 哈尔滨 150070;东北农业大学生命科学学院, 黑龙江 哈尔滨 150030;中国水产科学研究院黑龙江水产研究所, 淡水鱼类育种国家地方联合工程实验室, 黑龙江 哈尔滨 150070;中国水产科学研究院黑龙江水产研究所, 淡水鱼类育种国家地方联合工程实验室, 黑龙江 哈尔滨 150070;中国水产科学研究院黑龙江水产研究所, 淡水鱼类育种国家地方联合工程实验室, 黑龙江 哈尔滨 150070 |
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| 基金项目： | 国家“八六三”高技术研究发展计划（2011AA100402）；中央级公益性科研院所基本业务费专项（HSY201303）；农业部引进项目（2011-G12） |
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| 摘 要： | 为了克服单个家系数量性状位点(QTL)检测效率低、假阳性高等缺点,实验利用250对微卫星(SSR)标记对镜鲤8个全同胞家系的522尾子代进行基因组扫描,采用半同胞家系的分析策略对镜鲤体长(SL)和体质量(BW)性状进行QTL分析。结果显示,基于父系的QTL分析,共检测到4个QTL区间,其中,3个体长的QTL中,1个为95%基因组水平(genome-wide)显著性,位于LG24,可解释表型变异率为20.3%;其余2个均为95%染色体水平(chromosome-wide)显著性,分别位于LG6和LG30,可解释表型变异率分别为11.9%和11.6%。1个体质量的QTL达到99%基因组水平,位于LG24,可解释表型变异率达到38.3%,且与体长QTL区间重叠。基于母系的QTL分析,共检测到8个QTL区间,其中,5个体长的QTL中,1个为99%染色体水平,位于LG8,可解释表型变异率为16.6%;其余4个均为95%染色体水平,分别位于LG24、LG30、LG31和LG45,可解释表型变异率为9.6%~14.2%,且位于LG24和LG30上的QTL为父母本共有;3个体质量的QTL均与体长QTL区间重叠,1个为95%染色体水平,位于LG24,其余2个均为99%染色体水平,位于LG30和LG45,可解释表型变异率分别为14.1%和13.6%。进一步分析发现,位于LG24上的体长和体质量QTL区间重叠且均为父母本共有,体质量的3个QTL均与体长QTL存在重叠区域且呈现成簇分布的特点。本研究结果不仅可以为鲤分子育种提供更可靠的标记,而且为家系和品种间QTL变异规律的探索提供基础数据。
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| 关 键 词： | 镜鲤 多家系 体长 体质量 QTL |
| 收稿时间： | 2014/4/24 0:00:00 |
| 修稿时间： | 2014/6/23 0:00:00 |
Quantitative trait loci analysis for standard length and body weight in multi-families of mirror carp(Cyprinus carpio) |
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| ZHENG Xianhu,KUANG Youyi,LV Weihu,CAO Dingchen,DONG Fang and SUN Xiaowen.Quantitative trait loci analysis for standard length and body weight in multi-families of mirror carp(Cyprinus carpio)[J].Journal of Fisheries of China,2014,38(9):1263-1269. |
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| Authors: | ZHENG Xianhu KUANG Youyi LV Weihu CAO Dingchen DONG Fang and SUN Xiaowen |
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| Institution: | Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, National Local Joint Engineering Laboratory for Freshwater Fish Breeding, Harbin 150070, China;Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, National Local Joint Engineering Laboratory for Freshwater Fish Breeding, Harbin 150070, China;College of Life Science, Northeast Agricultural University, Harbin 150030, China;Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, National Local Joint Engineering Laboratory for Freshwater Fish Breeding, Harbin 150070, China;Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, National Local Joint Engineering Laboratory for Freshwater Fish Breeding, Harbin 150070, China;Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, National Local Joint Engineering Laboratory for Freshwater Fish Breeding, Harbin 150070, China |
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| Abstract: | Common carp(Cyprinus carpio)is one of the most widespread freshwater teleost species in the world.It has been cultured as an important food fish worldwide,especially in China,for several thousand years.In the past decade,much research efforts have been made for the molecular breeding of common carp including development of polymorphic markers,linkage mapping,and quantitative trait loci(QTL)analysis.However,QTL researches of common carp have been limited by single family and small population size.Considering the ability to detect and identify QTL in single family is often limited and has obtained false positive locus.We conducted a whole genome scan on 522 progeny from 8 full-sib families using 250 microsatellites selected from high density genetic linkage map of common carp constructed by our lab.The genetic maps were constructed by use of the Cri-map program with genotypes of 8 families,and genetic distances were estimated by use of the Kosambi map function.A total of 233 markers were organized to 47 linkage groups and the linkage maps covered a genetic distance of 3 131.5 cM,with the average interval for markers within linkage group of 16.8 cM.The linkage map could be used for primary QTL analysis.QTL identification of standard length(SL)and body weight(BW)traits was carried out using half-sib mapping strategies by GridQTL software.We obtained 4 QTL distributed across 3 linkage groups(LG)during sire-based QTL analysis.For SL,3 QTL were identified,of which 1 QTL occurred at the 95% genome-wide level,and was located on LG24,accounting for 20.3% of phenotype variation.The remaining 2 QTL were at the 95% chromosome-wide level,explaining 11.9%(LG6)and 11.6%(LG30),respectively.For BW,1 QTL was identified at 99% genome-wide level,explaining 38.3% of phenotypic variance and overlapped with the SL QTL intervals on LG24.During dam-based QTL analysis,we identified 8 QTL that were distributed across 5 LGs.Five QTL were associated with SL,of which one was at 99% chromosome-wide level and located at LG8.The other 4 QTL were at the 95% chromosome-wide level,accounting for 9.6%-20.3% of phenotypic variance.QTLs on LG24 and LG30 were significant both the sire and the dam-based analysis.For BW,three QTL were detected and have a similar confidence interval with SL at LG24,LG30 and LG45.Among these,2 QTL were identified at the 99% chromosome-wide level,and 1 QTL at the 95% chromosome-wide level,explaining 10.8%-14.1% of phenotypic variance.The results showed that the most significant QTLs for SL and BW were located on LG24 and common to both sire and dam.The results of this study not only can supply more reliable markers for molecular breeding of common carp,but also provide reference data for exploring regularity of QTL variation among different populations and families. |
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| Keywords: | Cyprinus carpio multi-family standard length body weight QTL |
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