| 一种简便的南瓜杂交种纯度SSR鉴定方法 |
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| 引用本文： | 韩小霞,粟建文,胡新军,袁祖华,李勇奇,杨建国.一种简便的南瓜杂交种纯度SSR鉴定方法[J].中国农学通报,2014,30(7):194-198. |
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| 作者姓名： | 韩小霞 粟建文 胡新军 袁祖华 李勇奇 杨建国 |
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| 作者单位： | 1. 湖南省蔬菜研究所2. 湖南省蔬菜工程技术研究中心 |
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| 基金项目： | 农业部公益性行业（农业）科研专项“南瓜产业技术研究与示范”(201303112)；“十二五”农村领域国家科技计划“南瓜杂种优势利用与新品种选育”(2012BAD02B03-17)。 |
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| 摘 要： | 针对目前南瓜杂交种纯度SSR鉴定方法操作繁琐，难以用于生产实践的问题，笔者介绍了一种简便的南瓜杂交种纯度SSR检测方法。该方法的基因组提取过程不需研磨、冷冻、离心、晾干等操作步骤，检测周期小于3 min，PCR反应体系与聚丙烯酰胺凝胶电泳的银染步骤，比常规方法更节约时间和成本，整个检测周期小于4 h，每人每天可完成480个样品的检测，且结果与田间鉴定结果吻合率达到99%。该方法可为南瓜杂交种的大规模快速纯度鉴定提供依据。
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| 关 键 词： | 发展思路 发展思路 |
| 收稿时间： | 2013/6/24 0:00:00 |
| 修稿时间： | 7/3/2013 12:00:00 AM |
A Simple SSR-based Method for Purity Identification of Cucurbita moschata Hybrid |
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| Abstract: | Because of the exsiting Cucurbita moschata hybrid purity identification system based on SSR was complicated and difficult for the production of practice, a simple Cucurbita moschata hybrid purity identification system based on SSR was introduced here. Without grinding, freezing, centrifugation and drying, the whole genomic DNA extraction period is less than 3 minutes. Comparing to the conventional method, the PCR detection system and the silver stained steps saved time and cost, the whole process is less than 4 hours, one person a day can complete 480 hybrids detection easily, and the coincidence rate of the simple SSR-based purity with the field test results was 99%. This method would provide the base for large-scale rapid purity identification of Cucurbita moschata hybrids. |
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| Keywords: | Purity identification |
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