| Detection of Beta-Lactamase and Extended-Spectrum Beta-Lactamase of Pathogens Isolated from Pig and Chicken and Their Antibiotic Susceptibility Test |
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| 作者姓名： | HU Gong-zheng ZHANG Chun-hui YUAN Li YANG Yu-rong LIANG Jun |
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| 作者单位： | [1]Faculty of Animal Husbandary and Veterinary Science, Henan Agricultural University, Zhengzhou 450002, P.R.China [2]Zhengzhou College of Animal Husbandary Engineering, Zhengzhou 450006, P.R.China |
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| 基金项目： | This study was supported by the National Natural Science Foundation of China （30471307）. |
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| 摘 要： | The antibacterial activity of beta-lactam antibiotics or their combinations with inhibitor sulbactum against non-lactamase- producing strains, lactamase-producing and ESBLs-producing isolates was evaluated with twofold dilution method after pathogens isolated from pigs and chickens were detected, respectively, for beta-lactamase and extended-spectrum beta- lactamases （ESBLs）, The results revealed that most of 43 clinically isolated strains could produce beta-lactamase and 3 strains of shigella isolated from chicken samples produced ESBLs. All of 30 lactamase-producing strains isolated and only one of 16 non-lactamase-producing strains were resistant to amoxicillin and ampicillin. MICs of ampicillin against lactamaseproducing isolates decreased 10-40 and 10-20 times respectively, when it was conbined with sulbactam at ration of 1：2 and 1：4. All clinical isolates were susceptible to third-generation cephalosporins. The MICs of third-generation cephalosporins against lactamase-producing isolates did not change when they were conbined with sulbactam. MICs of ceftiofur and ceftriaxone against ESBLs-producing isolates decreased 2-4 times when they were conbined with sulbactam.
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| 关 键 词： | 内酰胺酶 抗菌性能 鸡 猪 病原体 抗生素 |
Detection of Beta-Lactamase and Extended-Spectrum Beta-Lactamase of Pathogens Isolated from Pig and Chicken and Their Antibiotic Susceptibility Test |
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| HU Gong-zheng ZHANG Chun-hui YUAN Li YANG Yu-rong LIANG Jun.Detection of Beta-Lactamase and Extended-Spectrum Beta-Lactamase of Pathogens Isolated from Pig and Chicken and Their Antibiotic Susceptibility Test[J].Agricultural Sciences in China,2005,4(11):877-882. |
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| Authors: | HU Gong-zheng ZHANG Chun-hui YUAN Li YANG Yu-rong LIANG Jun |
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| Abstract: | The antibacterial activity of beta-lactam antibiotics or their combinations with inhibitor sulbactum against non-lactamaseproducing strains, lactamase-producing and ESBLs-producing isolates was evaluated with twofold dilution method after pathogens isolated from pigs and chickens were detected, respectively, for beta-lactamase and extended-spectrum betalactamases (ESBLs). The results revealed that most of 43 clinically isolated strains could produce beta-lactamase and 3strains of shigella isolated from chicken samples produced ESBLs. All of 30 lactamase-producing strains isolated and only one of 16 non-lactamase-producing strains were resistant to amoxicillin and ampicillin. MICs of ampicillin against lactamaseproducing isolates decreased 10-40 and 10-20 times respectively, when it was conbined with sulbactam at ration of 1:2 and 1:4. All clinical isolates were susceptible to third-generation cephalosporins. The MICs of third-generation cephalosporins against lactamase-producing isolates did not change when they were conbined with sulbactam. MICs of ceftiofur and ceftriaxone against ESBLs-producing isolates decreased 2-4 times when they were conbined with sulbactam. |
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| Keywords: | Beta-lactamase Extended-spectrum beta-lactamase Inhibitor Antibiotic susceptibility test |
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