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| 饲料中添加溶菌酶对草鱼生长性能和抗感染能力的影响 | |
| 引用本文： | 邢思华,华雪铭,朱站英,沈彦萍,王军,于宁,沈文婧.饲料中添加溶菌酶对草鱼生长性能和抗感染能力的影响[J].华中农业大学学报,2012,31(6):731-736. |
| 作者姓名： | 邢思华 华雪铭 朱站英 沈彦萍 王军 于宁 沈文婧 |
| 作者单位： | 1. 上海海洋大学水产与生命学院/农业部淡水水产种质资源重点实验室,上海,201306 2. 上海海洋大学水产与生命学院/农业部淡水水产种质资源重点实验室,上海201306 ;杭州海皇饲料开发有限公司,杭州311100 3. 上海沈李科工贸有限公司,上海,200436 4. 上海海洋大学水产与生命学院/农业部淡水水产种质资源重点实验室,上海201306; 南京市溧水县农业局,溧水211200 |
| 基金项目： | 上海市教委创新项目（10YZ126）、上海高校创新团队（第二期）建设项目和上海市宝山区科学技术委员会产学研合作项目 |
| 摘 要： | 在基础饲料中分别添加溶菌酶制品0(对照)、100、200、300、400和500mg/kg,每组设置5个重复,饲喂草鱼(6.93±0.27g)60d,旨在根据草鱼的生长性能和抗感染能力评价溶菌酶的添加效果,并确定其适宜用量。结果表明,饲料中添加溶菌酶对草鱼生长性能和抗感染能力有显著影响。试验前45d,试验组的草鱼特定生长率显著低于对照组;60d时,100～300mg/kg组的草鱼特定生长率与对照组差异不显著,400mg/kg组和500mg/kg组显著低于对照组。各试验组草鱼的饲料系数随饲料中溶菌酶含量的增加呈现先降低再升高的趋势,200mg/kg组饲料系数最低。通过腹腔注射的方法对草鱼进行嗜水气单胞菌攻毒,结果表明,200～500mg/kg组的草鱼死亡率显著低于对照组和100mg/kg组。饲用溶菌酶对草鱼内源免疫酶活性和内源消化酶活性有一定的刺激作用。抑菌试验结果表明,该溶菌酶制品对常见革兰氏阴性菌有明显的抑菌作用。综合草鱼的生长性能和抗感染能力,认为该试验条件下溶菌酶制品在草鱼饲料中的适宜添加量为200～300mg/kg。 |
| 关 键 词： | 溶菌酶 草鱼 生长性能 抗感染 内源酶 |
| 收稿时间： | 2012/3/27 0:00:00 |
Effect of dietary lysozyme levels on growth and anti-infection ability of Ctenopharyngodon idellus |
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| XING Si-hua , HUA Xue-ming , ZHU Zhan-ying , SHEN Yan-ping , WANG Jun , YU Ning , SHEN Wen-jing.Effect of dietary lysozyme levels on growth and anti-infection ability of Ctenopharyngodon idellus[J].Journal of Huazhong Agricultural University,2012,31(6):731-736. | |
| Authors: | XING Si-hua HUA Xue-ming ZHU Zhan-ying SHEN Yan-ping WANG Jun YU Ning SHEN Wen-jing |
| Institution: | 1.Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture/ Shanghai Ocean University,College of Fisheries and Life Science,Shanghai 201306,China; 2.Shanghai Shenli Technology-Industry-Trade Co.Ltd,Shanghai 200436,China; 3.Hangzhou Haihuang Feed Development Co.Ltd,Hangzhou 311100,China; 4.Bureau of Agriculture of Lishui County in Nanjing,Lishui 211200,China |
| Abstract: | To evaluate the effect of dietary lysozyme on growth and anti-infection ability of grass carp,Ctenopharyngodon idellus,fish with initial body weight 6.93±0.27 g were randomly divided into six groups and fed diets with 0 (control),100,200,300,400,500 mg/kg dietary lysozyme respectively for 60 days.The results showed that the growth performance and anti-infection ability of grass carp were significantly affected by dietary lysozyme.In the first 45 days of the feeding trial,the specific growth rate (SGR) in all lysozyme-treated groups was significantly lower than that in the control group (P<0.05).At the end of the feeding trial,the SGR in the 100-300 mg/kg groups was not significantly different from the control,while the SGR in the 400 and 500 mg/kg groups was significantly lower than that in the control (P<0.05).Feed conversion ratio decreased first,then increased with the increment of the dietary lysozyme,with the lowest in the group of 200 mg/kg.After the tested fish was challenged with live Aeromonas hydrophila by intraperitoneal injection,mortality rate of the 200-500 mg/kg groups was significantly lower than that of the control and 100 mg/kg group.This study further indicated that activities of endogenous digestive and immune enzymes were enhanced by the dietary lysozyme to some extent.Lysozyme was also found to have strong inhibitory activity against common Gram negative bacteria in aquaculture by antimicrobial tests.In summary,the optimal level of dietary lysozyme was 200-300 mg/kg according to the growth performance and the anti-infection ability. |
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