| 食源性大肠杆菌的PCR检测 |
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| 引用本文： | 席美丽,只 帅,王小璞,杨保伟,王 新.食源性大肠杆菌的PCR检测[J].西北农业学报,2011,20(12):188-181. |
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| 作者姓名： | 席美丽 只 帅 王小璞 杨保伟 王 新 |
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| 作者单位： | （西北农林科技大学 食品学院，陕西杨凌 712100） |
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| 基金项目： | 西北农林科技大学校长基金。 |
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| 摘 要： | 为了将PCR技术更好地应用于食品安全检测，利用多重PCR对大肠杆菌uidA基因和7种毒理基因进行扩增，并对人为污染大肠杆菌的水和牛奶进行普通和实时定量PCR限度检测。结果发现，2组多重PCR反应即可完成uidA和7种毒理基因的检测；普通PCR和实时定量PCR检测限度相同，对人为污染水检测限度为2.8×10 cfu/mL， 对人为污染牛奶的检测限度为2.8×104 cfu/mL。
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| 关 键 词： | PCR 大肠杆菌 毒力基因 限度检测 |
PCR Detection of Food borne E. coli |
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| XI Meili,ZHI Shuai,WANG Xiaopu,YANG Baowei and WANG Xin.PCR Detection of Food borne E. coli[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(12):188-181. |
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| Authors: | XI Meili ZHI Shuai WANG Xiaopu YANG Baowei and WANG Xin |
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| Abstract: | In order to apply PCR technology in the food safety, a multiple PCR protocol was developed to detect pathogenic E. coliin food. Primers for uidA and seven virulence genes were designed, and the detection limit of E. coliwere determined in artificially contaminated water and milk using general PCR and real time PCR. It was demonstrated that two multiple PCRs can identify seven virulence gene and uidA gene for pathogenic E. coli. The detection limits of general PCR and real time PCR test were the same: for artificially contaminated water, the detection limit was 2.8 × 10 cfu/mL; for contaminated milk, the detection limit was 2.8 × 104 cfu/mL. |
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| Keywords: | PCR E coli Virulence gene Detection limit |
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