| 湖南耐寒赤桉工厂化育苗组培快繁技术研究 |
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| 引用本文： | 饶红欣,蒋利媛,陈灵,罗先权,刘先芳.湖南耐寒赤桉工厂化育苗组培快繁技术研究[J].湖南林业科技,2002,29(2):4-7. |
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| 作者姓名： | 饶红欣 蒋利媛 陈灵 罗先权 刘先芳 |
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| 作者单位： | 湖南省森林植物园,湖南,长沙,410116 |
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| 摘 要： | 研究结果表明：MS＋BAO．2＋IBA1．0为最佳继代培养基，MS＋BAO．1＋OBA0．5为最佳壮苗培养基；MS＋IBA1．0为最佳生根培养基，组培过程中，应以MS为基本培养基，且激素BA用量不宜超过1．0mg/L；以自然光为辅助光源可提高耐寒赤桉试管苗的生根率和生根质量；半固体培养基由于会引起组培材料褐变而不宜使用，液体培养基对芽增殖，高生长与生根均有促进；季节姝选择地试管苗移栽成活率影响很大，选择5月份移栽，成活率可达96．0％以上。
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| 关 键 词： | 工厂化育苗 快繁技术 赤桉 耐寒性 试管苗 组织培养 |
Study on the technology of factory high-speed breeding and tissue culture of cold-resistant Eucalyptus in Hunan Province |
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| RAO Hong-xin,JIANG Li-yuan,CHEN Ling,LUO Xian-quan,LIU Xian-fang.Study on the technology of factory high-speed breeding and tissue culture of cold-resistant Eucalyptus in Hunan Province[J].Hunan Forestry Science & Technology,2002,29(2):4-7. |
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| Authors: | RAO Hong-xin JIANG Li-yuan CHEN Ling LUO Xian-quan LIU Xian-fang |
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| Abstract: | Research results:MS+BA0 2+IBA1 0 is the best step generational culture medeum;MS+BA0 1+IBA0 5 is the best strong sprout culture medium,MS+IBA1 0 is the best strike root culture medium.During the tissue culture,MS should be the base tissue medium and the quantity of hormone AB should be less than 1 0mg/L.The natural light used for auxiliary light can accelerate the rate and quality of strike root of test-tube breeding of Eucalyptus.The semi-solid tissue medium was not suitable because it may cause the tissue culture to turn brown;the liquid tissue medium can accelerate the development of bud,growth and stride root.The method is effective for transplantation the test-tube culture The survival rate can increase 96% by transplanting in May. |
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| Keywords: | Eucalyptus cold-resistant high-speed breeding and tissue culture test-tube breeding |
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