| 稻田养殖沙塘鳢对稻田水体及底泥微生物群落结构及多样性的影响 |
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| 引用本文： | 赵翔刚,罗衡,刘其根,赵良杰,蔡林荣,戴亮亮,张真.稻田养殖沙塘鳢对稻田水体及底泥微生物群落结构及多样性的影响[J].淡水渔业,2017,47(4):8-14. |
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| 作者姓名： | 赵翔刚 罗衡 刘其根 赵良杰 蔡林荣 戴亮亮 张真 |
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| 作者单位： | 上海海洋大学,农业部水产种质资源与利用重点开放实验室,上海 201306 |
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| 基金项目： | 公益性行业(农业)专项:淡水池塘工程化改造与环境修复技术研究与示范，上海市高校知识服务平台项目 |
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| 摘 要： | 为了研究养殖沙塘鳢(Odontobutis obscurus)对稻田水体及底泥的微生物群落结构及多样性的影响,2015年于浙江海盐江南四阡现代农业公司进行了沙塘鳢的稻田养殖实验,利用DGGE(Denaturing Gradient Gel Electrophoresis)技术对养殖过程中的稻田水体及底泥中细菌的16S r DNA片段进行指纹图谱分析。DGGE条带测序分析结果显示:稻田水体及底泥共检测到包括α-变形菌亚门(Alphaproteobacteria)、β-变形菌亚门(Betaproteobacteria)、γ-变形菌亚门(Gammaproteobacteria)、δ-变形菌亚门(Deltaproteobacteria)、ε-变形菌亚门(Epsilonproteobacteria)、绿菌门(Chlorobi)、酸杆菌门(Acidobacteria)、厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)、蓝藻门(Cyanobacteria)等细菌门类。多样性分析结果显示,养殖沙塘鳢的稻田底泥微生物DGGE条带数多于常规养殖稻田,养殖稻田的底泥Shannon多样性指数2.86,显著高于常规稻田的2.27,同时养殖稻田养殖沟底泥的Shannon多样性指数随养殖时间由2.56变化到2.16。PCA(Principal Component Analysis)及DGGE聚类分析结果显示,养殖稻田的水体及底泥微生物群落结构与常规稻田存在较大差异。结果表明,稻田养殖环境的微生态条件可能优于常规稻田。
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| 关 键 词： | 稻田养鱼 DGGE 沙塘鳢(Odontobutis obscurus) |
Influence of the cultured Odontobutis obscurus to the microbial community structure and diversity in rice-fish system |
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| ZHAO Xiang-gang,LUO Heng,LIU Qi-gen,ZHAO Liang-jie,CAI Lin-rong,DAI Liang-liang,ZHANG Zhen.Influence of the cultured Odontobutis obscurus to the microbial community structure and diversity in rice-fish system[J].Freshwater Fisheries,2017,47(4):8-14. |
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| Authors: | ZHAO Xiang-gang LUO Heng LIU Qi-gen ZHAO Liang-jie CAI Lin-rong DAI Liang-liang ZHANG Zhen |
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| Abstract: | In order to study influence of the cultured Odontobutis obscurus to the microbial community structure and diversity in rice-fish system.We investigated the microbial diversity of the water and sediment in rice-fish system using DGGE technology in Haiyan,Zhejiang.In this study,we detected species of Alphaproteo bacteria,Betaproteo bacteria,Gammaproteo bacteria,Deltaproteo bacteria,Epsilonproteo bacteria,Chlorobi,Acido bacteria,Firmicutes,Actino bacteria,Cyano bacteria.Diversity analysis showed that the number of sediment microbial species in rice-fish system were higher than that in the conventional rice fields.The Shannon-Weiner index in sediment of the rice-fish system was 2.86,significantly higher than 2.27 of the conventional rice field The Shannon-Weiner index in sediment was declined from 2.56 to 2.16 along the cultured process in rice-fish system.PCA and DGGE results showed the obvious differences of the microbial community structure in water and sediment between the rice-fish system and the conventional paddy fields,which indicated the cultured species O.obscurus may play an important role in the regulation of the microbial community structure. |
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| Keywords: | rice-fish system DGGE Odontobutis obscurus |
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