| 阿维菌素亚致死剂量下2种色型豌豆蚜解毒酶活力的研究 |
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| 引用本文： | 王小强,刘长仲.阿维菌素亚致死剂量下2种色型豌豆蚜解毒酶活力的研究[J].中国生态农业学报,2014,22(6):675-681. |
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| 作者姓名： | 王小强 刘长仲 |
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| 作者单位： | 甘肃农业大学草业学院/草业生态系统教育部重点实验室/中 美草地畜牧业可持续发展研究中心 兰州 730070;甘肃农业大学草业学院/草业生态系统教育部重点实验室/中 美草地畜牧业可持续发展研究中心 兰州 730070 |
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| 基金项目： | 国家自然科学基金项目(31260433)和高等学校博士学科点专项科研基金博导类课题(20136202110007)资助 |
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| 摘 要： | 采用室内培养方法研究了阿维菌素亚致死剂量分别处理红色和绿色型豌豆蚜后,其乙酰胆碱酯酶(AChE)、谷胱甘肽S-转移酶(GSTs)和多功能氧化酶(MFO)比活力在不同时间内的变化。结果表明,亚致死剂量LC20、LC10和LC5分别处理2种色型豌豆蚜24 h后,3种酶比活力均高于对照,且GSTs和MFO比活力均随亚致死剂量升高而增大,但AChE比活力表现为2种色型均在LC10处理后达到最大值,分别为13.93 nmol·min-1·mg-1(pro)和53.93nmol·min-1·mg-1(pro)。各处理表现为AChE比活力绿色型大于红色型,GSTs比活力与前者相反,而MFO比活力红、绿色型间无明显规律。随着处理后取样时间的变化,对红、绿色型豌豆蚜AChE、GSTs和MFO比活力的影响均表现为诱导或抑制作用,其中,GSTs比活力绿色型在0 h均达到最大值nmol·min-1·mg-1(pro)]且显著高于其余时间,分别为49.84(CK)、48.87(LC5)、42.74(LC10)和45.35(LC20)。2种色型豌豆蚜体内AChE、GSTs和MFO比活力变化不但受阿维菌素不同亚致死剂量的影响,还表现出明显的时间效应。
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| 关 键 词： | 豌豆蚜 色型 阿维菌素 亚致死剂量 解毒酶 |
| 收稿时间： | 2014/1/20 0:00:00 |
| 修稿时间： | 3/7/2014 12:00:00 AM |
Detoxification enzymes activities in two color morphs of pea aphid (Acyrthosiphon pisum) treated with different sub-lethal concentrations of avermectin |
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| WANG Xiaoqiang and LIU Changzhong.Detoxification enzymes activities in two color morphs of pea aphid (Acyrthosiphon pisum) treated with different sub-lethal concentrations of avermectin[J].Chinese Journal of Eco-Agriculture,2014,22(6):675-681. |
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| Authors: | WANG Xiaoqiang and LIU Changzhong |
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| Institution: | College of Pratacultural Science, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem, Ministry of Education/Sino-U.S. Center for Grazingland Ecosystem Sustainability, Lanzhou 730070, China;College of Pratacultural Science, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem, Ministry of Education/Sino-U.S. Center for Grazingland Ecosystem Sustainability, Lanzhou 730070, China |
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| Abstract: | Pea aphid, Acyrthosiphon pisum (Harris) (Hemiptera: aphidiae), is an important agricultural pest for a wide range of leguminous crops with a very strong reproductive capacity. Pea aphid is a well-studied species in terms of color polymorphism, which occurs as two distinct (red and green) color morphs. It has been proposed that the occurrence of this color polymorphism was a response to environmental factors (in particular pesticides) which increase the adapting ability of A. pisum to changed environment. The resistance of pea becomes weak or disappeared as pea exposed to red color morphs, indicating biological type characters of A. pisums. To learn pesticides effects on different color morphs of A. pisum, the specific activities of acetylcholinesterase (AChE), glutathione S-transferase (GSTs) and mixed function oxidase (MFO) of red and green morphs of A. pisum treated with different sub-lethal concentrations of avermectin were studied in this paper. The results showed that the specific activities of AChE, GSTs and MFO of red and green morphs of A. pisum treated with different concentrations (LC20, LC10 and LC5) of avermectin were higher than those of the control after 24 h. Also the specific activities of GSTs and MFO improved with increasing sub-lethal dosage. However, the specific activity of AChE of red and green morphs ofA. pisum reached maximum values of 13.93 nmol·min-l·mg-l(pro) and 53.93 nmol·min-l·mg-l(pro), respectively, under LC10. The specific activity of AChE of green morph of A. pisum was higher than of red morph, and GSTs showed the reverse trend. However, no significant trends were noted in specific activity of MFO between red and green morphs of A. pisum treated with different concentrations. The specific activities of AChE, GSTs and MFO of red and green morphs of A. pisum treated with LC20, LC10 and LC5 of avermectin decreased or increased with time. Specially, the specific activity of GSTs of green morph of A. pisum treated with different concentrations of avermectin reached the maximum values which were 49.84 nmol·min-l·mg-l(pro) for CK, 48.87 nmol·min-l·mg-l(pro) for LC5, 42.74 nmol·min -l·mg -l(pro) for LC10 and 45.35 nmol·min -l·mg -l(pro) for LC20] at 0 h and were significantly higher than those of the rest of the other time scales. In summary, the change in specific activities of AChE, GSTs and MFO of red and green morphs of A. pisum had obvious links with treated concentrations and time. |
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| Keywords: | Acyrthosiphon pisum Color morph Avermectin Sublethal concentration Detoxification enzyme |
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