| 水稻白叶枯病菌harpin基因的克隆与表达 |
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| 引用本文： | 闻伟刚,王金生.水稻白叶枯病菌harpin基因的克隆与表达[J].植物病理学报,2001,31(4):295-300. |
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| 作者姓名： | 闻伟刚 王金生 |
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| 作者单位： | 南京农业大学植物保护学院, 农业部病虫监测与治理重点开放实验室, 南京 210095 |
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| 基金项目： | 国家高技术发展计划(863-101-03-02-01);国家重点基础研究发展计划(G2000016201)资助 |
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| 摘 要： | 用PCR方法从水稻黄单胞菌白叶枯致病变种(Xanthomonas oryzae pv.oryzae,Xoo) JxoIII菌株中克隆到编码诱导植物过敏性反应激发子的基因hrfA。同源性比较发现其推测产物与水稻白叶枯病菌菲律宾小种Pxo86的Hpa1有97.2%的序列一致性,比Hpa1少了一个-GGG-GG-氨基酸残基序列重复。基因经NdeI和BamHI双酶切后连到含T7启动子的高表达载体pET 30a (+)上构建重组质粒pHRF4,并转化宿主菌BL21(DE3)产生表达菌株BLHR4。经过IPTG诱导之后,BLHR4产生一分子量为15.6 kD的蛋白质。研究表明,该蛋白在性质与功能上类似于其它已发现的harpins,即能够在烟草上引起典型的过敏性反应,富含甘氨酸、热稳定以及对蛋白酶敏感。因此,我们把该蛋白定名为harpinXoo。harpinXoo还具有诱导植物抗病性的功能。
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| 关 键 词： | 水稻白叶枯病菌 过敏性反应 hrfA harpinXoo 诱导抗病性 |
| 文章编号： | 0412-0914(2001)04-0295-06 |
| 修稿时间： | 2001年5月14日 |
CLONING AND EXPRESSING A harpin GENE FROM Xanthomonas oryzae pv. oryzae |
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| WEN Wei gang,WANG Jin sheng.CLONING AND EXPRESSING A harpin GENE FROM Xanthomonas oryzae pv. oryzae[J].Acta Phytopathologica Sinica,2001,31(4):295-300. |
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| Authors: | WEN Wei gang WANG Jin sheng |
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| Institution: | Key Lab. of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture;Dept. of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | A hrfA gene which encode plant hypersensitive response elicitor was cloned from Xanthomonas oryzae pv. oryzae isolate JxoIII by PCR. It encodes a predicted 140 amino acids protein that is glycine rich. Homology research showed that the protein is 97.2% identical with the Hpa1 of Xanthomonas oryzae pv. oryzae (Pxo86), but the number of the GGG GG repeats is one less. In Escherichia coli , BLHR4 strain overexpressing hrfA , the 15.6 kD protein fractionated from E.coli has been identified as the harpin Xoo . Like other harpins, harpin Xoo eli cits a typical HR in tobacco leaves. It is heat stable and appear highly sensitive to protease. Moreover, harpin Xoo has the function of inducing plant disease resistance. |
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| Keywords: | Xanthomonas oryzae pv oryzae hypersensitive response hrfA harpin Xoo induced disease resistance |
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