| 离体诱变改良杂交水稻的研究(英文) |
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| 引用本文： | 蔡其华,高明尉,梁竹青.离体诱变改良杂交水稻的研究(英文)[J].核农学报,1990,4(3):169-174. |
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| 作者姓名： | 蔡其华 高明尉 梁竹青 |
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| 作者单位： | 浙江农业大学原子核农学科学研究所,浙江农业大学原子核农学科学研究所,浙江农业大学原子核农学科学研究所 杭州310029 ,杭州310029 ,杭州310029 |
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| 摘 要： | 用γ射线照射杂交籼稻保持系与恢复系的成熟胚、幼胚及愈伤组织,研究其离体培养反应。结果表明,幼胚在培养前经1kR照射处理能提高愈伤组织的诱导率和分化率。成熟胚的最佳照射量为2.5—5.0kR,而照射成熟胚的愈伤组织则以250R为佳。经照射处理的培养物,其后代体细胞无性系的变异频率显著提高。通过离体诱变方法已选得10份有希望材料,其中8份保持系和2份恢复系。
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| 关 键 词： | 离体培养 诱变 水稻 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
In vitro CULTURE MUTAGENESIS FOR HYBRID RICE IMPROVEMENT |
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| Abstract: | The mutagenic effect of gamma-radiation on culturability and so-maclonal variation in hybrid rice in vitro culture was investigated. The results indicated that 1 kR applied to immature embryo led to a higher rate of callusing and plantlet regeneration. 2.5-5.0 kR would be the appropriate exposure range for mature emdryo, while 250 R was the optimum dose for irradiating the callus derived from mature seeds. Application of proper exposure of gamma-rays to the rice cultures greatly enhanced the somaclonal variation frequency; in some cases, it went up to 81.5%. 10 promising lines including 8 maintainer and 2 restorer lines have been developed for field evaluation. |
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| Keywords: | in vitro culture mutagenesis rice |
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