| 爬山虎体细胞胚的发生及组织学研究 |
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| 引用本文： | 冯大领,李云,孙振元.爬山虎体细胞胚的发生及组织学研究[J].北京林业大学学报,2004,26(4):97-99,i002. |
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| 作者姓名： | 冯大领 李云 孙振元 |
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| 作者单位： | 北京林业大学生物科学与技术学院;中国林业科学研究院花卉研究中心 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 该文以爬山虎子叶的叶片和叶柄为试验材料 ,对爬山虎体细胞胚发生进行了研究 .结果表明 :爬山虎子叶在MS附加 6 BA 0 3mg L和NAA 0 1mg L培养基上或 1/ 2MS附加 6 BA 0 5mg L和NAA 0 1mg L培养基上都能诱导产生体细胞胚 ,子叶叶柄在 1/ 2MS附加 6 BA 0 3mg L的培养基上也产生了体细胞胚 .胚性愈伤组织转移到无植物生长调节剂的MS或B5培养基上均能发育成正常植株 .组织学观察表明 ,爬山虎体细胞胚的形成经历了球形胚、心形胚、鱼雷胚、子叶胚等几个阶段 ,逐渐发育成正常植株 ,这与合子胚的发育途径相似 .体细胞胚在发育过程中出现了连体胚、芽端畸形胚、超度含水状胚等几种畸形胚
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| 关 键 词： | 爬山虎 体细胞胚发生 组织学 |
Histology and somatic embryogenesis of Parthenocissus tricuspidata |
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| FENG Daling,LI Yun,SUN Zhenyuan.School of Biological Sciences and Biotechnology,Beijing Forestry University,P.R.China..Histology and somatic embryogenesis of Parthenocissus tricuspidata[J].Journal of Beijing Forestry University,2004,26(4):97-99,i002. |
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| Authors: | FENG Daling LI Yun SUN ZhenyuanSchool of Biological Sciences and Biotechnology Beijing Forestry University PRChina |
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| Institution: | FENG Daling,LI Yun,SUN Zhenyuan.School of Biological Sciences and Biotechnology,Beijing Forestry University,100083,P.R.China. |
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| Abstract: | Somatic embryogenesis of Parthenocissus tricuspidata is studied in this paper.The results show that somatic embryos are obtained from cotyledons of P.tricuspidata induced on MS medium containing 0.3 mg/L 6--BA and 0.1 mg/L NAA or 1/2MS medium containing 0.5 mg/L 6--BA and 0.1 mg/L NAA,and from cotyledon petioles induced on 1/2MS medium containing 0.3 mg/L 6--BA.Embryonic callus cells are developed to normal seedlings when subcultured on MS or B\-5 medium without plant growth regulator.Histological observations reveal that the somatic embryos successively differentiate through globular-shaped,heart-shaped,torpedo stages and cotyledon stages,similar to the development of zygotic embryo.There are several abnormal embryos including joined embryo,abnormal-bud embryo and hyperhydric embryo,etc.in the induction stage of somatic embryos. |
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| Keywords: | Parthenocissus tricuspidata somatic embryogenesis histology |
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