| 反相高效液相色谱法测定荠菜中的维生素C |
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| 引用本文： | 吴海燕,张新宇,陈建军,唐明霞,邱卫池,程玉静,袁春新.反相高效液相色谱法测定荠菜中的维生素C[J].中国果菜,2020(2):44-47,53. |
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| 作者姓名： | 吴海燕 张新宇 陈建军 唐明霞 邱卫池 程玉静 袁春新 |
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| 作者单位： | 南通科技职业学院;南通市农村专业技术协会;南通市市场监督管理局;江苏沿江地区农业科学研究所;江苏中宝食品有限公司 |
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| 基金项目： | 南通市民生科技重点项目（MS22018016） |
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| 摘 要： | 本试验以超声波辅助提取荠菜中VC,并采用反相高效液相色谱法测定其含量。先将荠菜匀浆后,以2%草酸为萃取溶剂,100 W的功率,25℃超声提取10 min,0.45μm微孔滤膜过滤,得到VC提取液,再进行反相高效液相色谱测定,以0.1%草酸溶液为流动相,流速1.0 mL/min,采用C18反相色谱柱分离,在246 nm波长定量检测。结果表明,超声提取荠菜中VC含量为56.05 g/100 g;VC浓度在2~20μg/mL范围内峰面积与VC浓度间线性良好,相关系数为0.9998;VC含量的检出限为0.6 mg/100 g,定量限为2 mg/100 g。方法回收率为99.73%~101.48%;重复性的相对标准偏差为1.97%。可见该方法能有效提取蔬菜中VC,测定结果稳定,重现性好,可以用于蔬菜中VC的提取与检测。
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| 关 键 词： | 超声波提取 反相高效液相色谱法 维生素C 荠菜 |
Determination of Vitamin C in Capsellae by RP-HPLC |
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| WU Hai-yan,ZHANG Xin-yu,CHEN Jian-jun,TANG Ming-xia,QIU Wei-chi,CHENG Yu-jing,YUAN Chun-xin.Determination of Vitamin C in Capsellae by RP-HPLC[J].China Fruit and Vegetable,2020(2):44-47,53. |
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| Authors: | WU Hai-yan ZHANG Xin-yu CHEN Jian-jun TANG Ming-xia QIU Wei-chi CHENG Yu-jing YUAN Chun-xin |
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| Institution: | (Nantong College of Science and Technology,Nantong 226007,China;Nantong Rural Professional Technique Association,Nantong 226006,China;Nantong Administration for Market Regulation,Nantong 226018,China;Jiangsu Yanjiang Institute of Agriculture Science,Nantong 226541,China;Jiangsu Zhongbao Food Co.,Ltd,Haimen 226200,China) |
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| Abstract: | In this experiment,VC in capsellae was extracted by ultrasonic assisted method,and its content was determined by RP-HPLC.Capsella after homogenate was extracted by ultrasonic assisted method with 2%oxalic acid as the extraction solvent,100 W power,25℃for 10 min.Then the samples were filtered through 0.45μm membrane,detected by RP-HPLC with Hypersil C18 column.Mobile phase was 0.1%oxalic acid with flow rate 1.0 mL/min,ultraviolet detection wavelength was at 246 nm.The results showed that the content of VC in capsella was 56.05 g/100 g.When the concentration of vitamin C were 2-20μg/mL,the linear relation between peak area and concentration of vitamin C were good.And the coefficient of correlation for the regression equation was 0.9998.The detection limits was 0.6 mg/100 g.The detection limits of quantification was 2 mg/100 g.The recovery for the vitamin C was in the range from 99.73%to 101.48%,with the relative standard deviation(RSD)was 1.97%.This method had good precision and high accuracy.This method can determine vitamin C in vegetables. |
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| Keywords: | Ultrasonic-assistant extraction RP-HPLC vitamin C capsella |
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