Genetic and agronomic traits stability of marker-free transgenic wheat plants generated from Agrobacterium-mediated co-transformation in T_2 and T_3 generations |
| |
| Authors: | LIU Hui-yun WANG Ke WANG Jing DU Li-pu PEI Xin-wu YE Xing-guo |
| |
| Institution: | 1 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China |
| |
| Abstract: | Genetically modified wheat has not been commercially utilized in agriculture largely due to regulatory hurdles associated with traditional transformation methods. Development of marker-free transgenic wheat plants will help to facilitate biosafety evaluation and the eventual environmental release of transgenic wheat varieties. In this study, the marker-free transgenic wheat plants previously obtained by Agrobacterium-mediated co-transformation of double T-DNAs vector were identified by fluorescence in situ hybridization(FISH) in the T_1 generation, and their genetic stability and agronomic traits were analyzed in T_2 and T_3 generations. FISH analysis indicated that the transgene often integrated into a position at the distal region of wheat chromosomes. Furthermore, we show that the GUS transgene was stably inherited in the marker-free transgenic plants in T_1 to T_3 generations. No significant differences in agronomic traits or grain characteristics were observed in T_3 generation, with the exception of a small variation in spike length and grains per spike in a few lines. The selection marker of bar gene was not found in the transgenic plants through T_1 to T_3 generations. The results from this investigation lay a solid foundation for the potential application of the marker-free transgenic wheat plants achieved through the co-transformation of double T-DNAs vector by Agrobacterium in agriculture after biosafty evaluation. |
| |
| Keywords: | wheat marker-free transgenic plants genetic stability |
| 本文献已被 CNKI ScienceDirect 等数据库收录！ |
| 点击此处可从《农业科学学报(英文版)》浏览原始摘要信息 |
| 点击此处可从《农业科学学报(英文版)》下载免费的PDF全文 |
