| 钩藤组培技术体系研究 |
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| 引用本文： | 施力军,苏建春,蒋向军,马小军,韦树根,蒋剑刚,覃吉胜,白隆华.钩藤组培技术体系研究[J].安徽农业科学,2010,38(6):2937-2937,2955. |
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| 作者姓名： | 施力军 苏建春 蒋向军 马小军 韦树根 蒋剑刚 覃吉胜 白隆华 |
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| 作者单位： | 广西药用植物园,广西南宁,530023;桂林亦元生现代生物技术有限公司,广西桂林,541004;广西药用植物园,广西南宁,530023;中国医学科学院,中国协和医科大学药用植物研究所,北京,100094 |
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| 基金项目： | 广西科技厅科技攻关项目(桂科攻0718002-3-6) |
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| 摘 要： | 目的]寻找快速获得整齐一致钩藤苗的方法。方法]以钩藤当年生枝条和带芽茎段为外植体进行组织培养试验。结果]最佳芽诱导培养基为MS＋6-BA0.2 mg/L＋NAA0.1 mg/L＋IBA0.2 mg/L,最佳增殖培养基为MS＋6-BA2.0 mg/L＋NAA0.2 mg/L,最佳生根培养基为1/2MS＋NAA2.0 mg/L＋活性炭0.03%。结论]该研究可为钩藤的产业化生产提供理论与技术支撑。
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| 关 键 词： | 钩藤 组织培养 技术 |
Study on the Tissue Culture Technique System of Uncaria rhynchopylla(Miq.) Jacks. |
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| SHI Li-jun et al.Study on the Tissue Culture Technique System of Uncaria rhynchopylla(Miq.) Jacks.[J].Journal of Anhui Agricultural Sciences,2010,38(6):2937-2937,2955. |
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| Authors: | SHI Li-jun |
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| Institution: | Guangxi Botanical Garden of Medicinal Plants;Nanning;Guangxi 530023 |
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| Abstract: | Objective]The research aimed to find out the method of getting uniform Uncaria rhynchopylla(Miq.) Jacks.seedlings quickly.Method]With the current-year branches and stem sections with buds of Uncaria rhynchopylla(Miq.) Jacks.as explants,the experiment on its tissue culture was made.Result]The optimum medium for inducing the bud differentiation of Uncaria rhynchopylla(Miq.) Jacks.was MS+0.2 mg/L 6-BA+0.1 mg/L NAA +0.2 mg/L IBA.The optimum multiplication medium was MS+2.0 mg/L 6-BA+0.2 mg/L NAA.And the opti... |
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| Keywords: | Uncaria rhynchopylla(Miq ) Jacks Tissue culture Technique |
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