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棉花区试品种的AFLP指纹图谱分析
引用本文:孙宁,付小琼,王秀玲,刘逢举,耿向阳,曲京武,乔铁军,杨付新.棉花区试品种的AFLP指纹图谱分析[J].中国农学通报,2012,28(15):36-41.
作者姓名:孙宁  付小琼  王秀玲  刘逢举  耿向阳  曲京武  乔铁军  杨付新
作者单位:1. 中国农业科学院棉花研究所,河南安阳455000;山东检验检疫技术中心,山东青岛266000
2. 中国农业科学院棉花研究所,河南安阳,455000
3. 山东检验检疫技术中心,山东青岛,266000
基金项目:中央级公益性科研院所基本科研业务专项
摘    要:为了探索AFLP分子标记在构建国家区试棉花品种指纹图谱上的优势,以2009年和2010年参加国家黄河流域棉花区试的B组常规棉、C组杂交棉共31份品种为材料,运用筛选确定的2对AFLP核心引物对这些品种进行分子标记分析,共获得44个多态性片段,其中多态性位点占总扩增位点的28.39%,利用44个多态性片段初步构建了这些品种的AFLP指纹图谱。聚类结果显示,在相似系数为0.92时,31份区试品种完全区分开;常规棉之间、杂交棉之间大多不同程度的优先聚集在一起。

关 键 词:新疆农业科学院土壤肥料与农业节水研究所  新疆农业科学院土壤肥料与农业节水研究所  乌鲁木齐830091  
收稿时间:2011/10/26 0:00:00
修稿时间:2011/12/20 0:00:00

AFLP Fingerprinting Analysis of 31 Cultivars Attending the National Regional Trial
Sun Ning , Fu Xiaoqiong , Wang Xiuling , Liu Fengju , Geng Xiangyang , Qu Jingwu , Qiao Tiejun , Yang Fuxin.AFLP Fingerprinting Analysis of 31 Cultivars Attending the National Regional Trial[J].Chinese Agricultural Science Bulletin,2012,28(15):36-41.
Authors:Sun Ning  Fu Xiaoqiong  Wang Xiuling  Liu Fengju  Geng Xiangyang  Qu Jingwu  Qiao Tiejun  Yang Fuxin
Institution:1(1Cotton Research Institute of CAAS,Anyang Henan 455000;2Shandong Technical Center for Inspection & Quarantine,Qingdao Shandong 266000)
Abstract:For exploring the advantage of AFLP molecular marker in constructing the cotton fingerprinting,31 cultivars of conventional cottons in team B,hybrid cottons in team C attending the 2009 and 2010 national regional trials were used for DNA fingerprinting.Two pairs of core AFLP primer were used for polymorphism analysis.Forty-four polymorphic bands were obtained which took 28.39% of total amplified bands.A primary DNA fingerprint database was established with 44 polymorphic bands.The cluster analysis showed that,all tested-cultivars could be completely identified at 0.92 similarity index level,and conventional cottons almost cluster together preferentially in different extent,so as hybrid cottons.
Keywords:cotton  national regional trail  AFLP  fingerprinting
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