Characterization and separation of bovine lymphocyte populations |
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| Authors: | H Buschmann S Pawlas |
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| Institution: | Institut für Medizinische Mikrobiologie, Infektions- und Seuchenmedizin, Tierärztliche Fakultät, Universität München, Federal Republic of Germany |
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| Abstract: | Bovine lymphocyte populations were characterized by surface markers, rosette-forming ability and behaviour towards mitogens. After pre-treatment with neuraminidase 16% of the bovine blood lymphocytes and 14% of the bovine spleen cells formed spontaneous (E) rosettes with sheep erythrocytes. About 20% EAC rosette-forming cells were detected among both cell populations. Protein A receptors were detectable among 8% of the blood lymphocytes and 26% of the spleen cells. Bovine lymphocytes responded to pokeweed mitogen (PWM), phytohemagglutinin (PHA) and concanavalin A (Con A). An enrichment of bovine B and T cells was obtained by E-rosette sedimentation (81–84% B cells) and by filtration through nylon fiber columns (51–65% T cells). The T cells obtained after nylon filtration still responded to the mitogens PHA, Con A and PWM. Enriched B-cell populations responded to bacterial lipopolysaccharide (LPS). After monocyte depletion the mitogenic response of blood lymphocytes was not influenced. |
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| Keywords: | Bovine lymphocytes lymphocyte surface markers mitogens rosette formation separation of lymphocytes B cells T cells Lymphocytes bovins marqueurs de surface mitogènes formation de rosettes séparation des lymphocytes cellules B cellules T |
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