| 一株溶磷细菌的分离、鉴定及其溶磷特性研究 |
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| 引用本文： | 孙 珊,黄 星,范宁杰,冯昭中,李会会,李顺鹏.一株溶磷细菌的分离、鉴定及其溶磷特性研究[J].土壤,2010,42(1):117-122. |
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| 作者姓名： | 孙 珊 黄 星 范宁杰 冯昭中 李会会 李顺鹏 |
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| 作者单位： | 南京农业大学生命科学学院,农业部环境微生物工程重点开放实验室,南京,210095 |
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| 基金项目： | 科技部平台建设项目，南京市科技发展计划项目 |
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| 摘 要： | 从作物根际土壤样品中筛选到一株溶 P 能力强的菌株 GJT-1,结合生理生化指标和16S rDNA 序列分析鉴定其为假单胞菌(Pseudomonas sp.).菌株 GJT-1 对磷酸三钙、磷酸铝、磷酸铁有一定的溶解能力,28℃培养到第 3 天对磷酸三钙液体培养基的溶 P 量可达 224.51 mg/L.该菌对开阳磷矿粉和宜昌磷矿粉的溶 P 量分别为 194.25 mg/L 和 120.59 mg/L.通过扫描电镜观察到接种活菌处理的磷矿粉表面凹凸且有黏性物质附着,表明菌株 GJT-1 可利用磷矿粉.
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| 关 键 词： | 溶磷菌 溶磷能力 有效磷 |
Studies on phosphorus solubilizing activity of a strain isolated from corp rhizosphere |
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| SUN Shan,HUANG Xing,FAN Ning-jie,FENG Zhao-zhong,LI Hui-hui,LI Shun-peng.Studies on phosphorus solubilizing activity of a strain isolated from corp rhizosphere[J].Soils,2010,42(1):117-122. |
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| Authors: | SUN Shan HUANG Xing FAN Ning-jie FENG Zhao-zhong LI Hui-hui LI Shun-peng |
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| Institution: | SUN Shan,HUANG Xing,FAN Ning-jie,FENG Zhao-zhong,LI Hui-hui,LI Shun-peng ( Key Lab of Microbiological Engineering of Agricultural Environment,Ministry of Agriculture,Microbiology Department,College of Life Science,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | Phosphobacterium GJT-1 was isolated from the rhizosphere of crop and was identified as Pseudomonas sp.,based on its physiological characteristics and 16S rDNA sequence analysis.Phosphate-dissolving activity was measured in solid media as well as in liquid media using different phosphate sources including tricalium phosphate,aluminium phosphate,ferric phosphate.When phosphobacterium GJT-1 was inoculated into NBRIP liquid culture,the release of maximal available soluble phosphorus reached to 224.51 mg/L after... |
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| Keywords: | Pseudomonassp Phosphate-solubilizing bacteria Pseudomonas sp Phosphate solubilizing activity Availale phosphorus |
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