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| 转基因豆粕调控元件在饲料加工中降解变化的初步研究 | |
| 引用本文： | 梁杉,梁宁,蒋继志,胡东升.转基因豆粕调控元件在饲料加工中降解变化的初步研究[J].华北农学报,2009,24(2). |
| 作者姓名： | 梁杉 梁宁 蒋继志 胡东升 |
| 作者单位： | 1. 河北大学,生命科学学院,河北,保定,071002 2. 河北大学,图书馆,河北,保定,071002 3. 河北省创新国际中学,河北,张家口,075000 |
| 基金项目： | 公益性行业(农业)科研专项基金，河北省自然科学基金 |
| 摘 要： | 为了对转基因植物源饲料的安全性提供依据,针对转基因豆粕中的CaMV35S启动子和NOS终止子设计引物,利用PCR技术研究加热、高压蒸汽、膨化以及制粒加工工艺对豆粕中启动子及终止子片段长度的影响.结果表明,加热处理对豆粕中启动子基因和终止子基因的片段长度均没有影响;但高压蒸汽处理对豆粕中对启动子基因和终止子基因的影响明显不同,启动子基因的246,165和101 bp这3个片段在处理后均可被检测到,而终止子基因只有125 bp的片段能被检测到,217 bp片段在处理后检测不到;对以豆粕为原料的饲料产品进行检测也得到相同的结果,即启动子基因较为稳定,终止子基因则较易被破坏. |
| 关 键 词： | 豆粕 启动子 终止子 饲料加工 |
Degradation of Non-target Genes During Feed Processes of Genetically Modified Soybean Meal |
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| LIANG Shan,LIANG Ning,JIANG Ji-zhi,HU Dong-sheng.Degradation of Non-target Genes During Feed Processes of Genetically Modified Soybean Meal[J].Acta Agriculturae Boreali-Sinica,2009,24(2). | |
| Authors: | LIANG Shan LIANG Ning JIANG Ji-zhi HU Dong-sheng |
| Institution: | 1.College of Life Sciences;Hebei University;Baoding 071002;China;2.Library of Hebei University;3.Innovation High School of Hebei;Zhangjiakou 075000;China |
| Abstract: | In order to privide some knowledge for transgenic plant feed,primeres were designed on transgenic soybean meal within CaMV35S promoter gene and NOS terminator,and effect of heat treatment,autoclave,and other processing technics on degradation of CaMV35S and NOS in soybean meal was investigated by polymerase chain reaction detection system in this experiment.The results showed that heat treatment had no effect on degradation of both CaMV35S and NOS.Autoclave treatment also did not affect degradation of CaMV3... |
| Keywords: | Soybean meal Promoter Terminator Feed processing |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |