The effect of arachidonic acid and its derivatives on superoxide release of neutrophils primed by TNFα |
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| Authors: | HUANG Tie-sheng |
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| Institution: | Guiyang Medical College, Guiyang 550001, China |
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| Abstract: | AIM: TO study the control mechanism of the developing of inflammation diseases, two inflammatory mediators, TNFa and arachidonic acid(AA) and its derivatives have been used as stimuli to induce human peripheral blood neutrophils to produce superoxide radicals. METHODS: The method of lucigeni - dependent light release has been used to exam the ability of releasing superoxide of neutrophils induced by the cooperation of TNFa and high class fatty acids as well as their derivatives. RESULTS: TNFa pretreatment markedly augmented the amount of superoxide produced in response to AA. Similarly, polyunsaturated fatty acids(PUFA) such as eicosapentaenoic acid, docosahexaenoic acid, linoleic acid, linlenic acid as well as monounsaturated fatty acid oleic acid were effected by TNFa. However, hydroxide (C20:4, n - 6 hydroxy) and hydroperoxide(C20:4, n - 6 hydroperoxy) of AA were not effected by TNFa. Leukotriene B4(LTB4) and PGE2 which are products of AA metabolism via the lipoxygenase and cyclooxygenase pathways respectively. The two major major of mediators are generated during an inflammatory response. The former acted on neutrophils in the generation of reactive oxygen species while the latter played an inhibition function. CONCLUSION: The complexity of the mediator network operating in various immune reactions and interaction between unsaturated lipids and cytokines may play an important role in disease processes and regulation of immune responses. |
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| Keywords: | Neutrophils Arachidonic acid Tumor necrosis factor Superoxide |
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