Effects of adrenomedullin on proliferation of vascular smooth muscle cells induced by urotensin Ⅱ |
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| Authors: | QI Yong-fen XIA Chun-fang CHEN Ya-hong XUE Lin PANG Yong-zheng TANG Chao-shu |
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| Institution: | Institute of Cardiovascular Research, the First Hospital, Peking University, Beijing 100034, China |
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| Abstract: | AIM:To observe the effect of adrenomedullin(ADM)on proliferation of vascular smooth muscle cells(VSMC) induced by urotensin Ⅱ(UⅡ). METHODS:DNA synthesis of cultured rat aortic VSMC was measured by 3H]-TdR incorporation. The activities of mitogen activated protein kinase(MAPK) were determined by isotope tagged with γ-32P]-ATP. RESULTS:UⅡ(10-8mol/L) significantly increased 3H]-TdR incorporation of VSMC and MAPK activities by 38%(P＜0.05) and 260%(P＜0.01) respectively compared with control group. Compared with UⅡ group, 10-10,10-9,10-8mol/L ADM decreased 3H]-TdR incorporation of VSMC by 7%(P＞0.05), 32%(P＜0.05)and 41%(P＜0.01),respectively, and diminished MAPK activities by 24%(P＞0.05), 32%(P＜0.05)and 36%(P＜0.05),respectively. CONCLUSION:ADM inhibits proliferation of VSMC induced by urotensin Ⅱ through inhibiting MAPK activation. |
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| Keywords: | Mitogens Protein kinases Muscle smooth vascular Urotensins Adrenomedullin |
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