Effect of chronic hypoxia on L-Arginine/nitric oxide pathway in rat pulmonary artery |
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| Authors: | CHEN Jing-jiong GONG Yong-sheng ZHEN Lu-zhen JIANG Zhong-sun TANG Chao-shu PANG Yong-zheng |
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| Institution: | 1. Laboratory of Cor Pulmonale, Wenzhou Medical College, Wenzhou 325003,China;
2. Cardiovascular Disease Institute of First Hospital, Beijing Medical University, Beijing 100034,China |
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| Abstract: | AIM: To study the effect of chronic hypoxia on L-Arginine/NO pathway in rat pulmonary artery. METHODS: Changes in pulmonary artery L-Arginine(L-Arg) transport, nitric oxide synthase (NOS) activity, plasma nitrite level and L-Arg level in HPH rats were investigated. RESULTS: (1) The mean pulmonary arterial pressure (mPAP) and weight ratio of right ventricle to left ventricle and septum (RV/LV+S) of HPH group were higher than those in control group (P＜0.01). (2) Plasma L-Arg level in HPH group was not significantly changed. (3) At low (0.2 mmol/L)or high(5.0 mmol/L)concentration of L-Arg, the velocity of L-Arg transport in HPH group was lower than that in control group (P＜0.05 or P＜0.01). (4) The activity of pulmonary artery tNOS, iNOS and cNOS in HPH group were increased by 38.0%, 32.8% and 53.0%, respectively (P＜0.01), compared with control group. (5) Plasma NO level of HPH group was decreased, which was negative correlation to mPAP and RV/LV+S (P＜0.01). CONCLUSION: The decrease of nitric oxide generation might result from L-Arg transport injury, while pulmonary artery tNOS, iNOS and cNOS activity were enhanced during chronic hypoxia. |
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| Keywords: | Nitric oxide Anoxia Hypertension pulmonary |
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